LipidLaunch™ Ionizable and Cationic Lipid Screening Library (96-Well)

The LipidLaunch™ Ionizable and Cationic Lipid Screening Library (96-Well) includes 126 ionizable cationic and cationic lipids to screen for use in lipid nanoparticle (LNP) formulations. The lipids are formulated at 25 mg/ml in ethanol for ease of addition to lipid mixtures for LNP formulation. Lipids selected for this screening library include FDA-approved excipients, as well as some of the most commonly cited lipids used in nucleic acid-cargo LNPs. In addition, this screening library contains a wide variety of ionizable cationic lipids, including branched tail, disulfide-containing, multi-tail non-disulfide, polymeric/dendrimer, and unsaturated lipids. LNPs containing these lipids in combination with a sterol lipid, a phospholipid, and a PEGylated lipid have been used to encapsulate mRNA, plasmid DNA, siRNA, and other nucleic acid cargo for intracellular delivery. Lipids in this library have also been incorporated into LNPs to deliver CRISPR sgRNA and Cas9 mRNA for genome editing in vivo.^1,2 This screening library can be used in conjunction with Cayman’s LipidLaunch™ Helper Lipid Set (Item No. 42831 large) to encapsulate mRNA, plasmid DNA, siRNA, and other nucleic acid cargo for intracellular delivery. For more information, please see Cayman's LNP guide: Lipid Nanoparticle Formulation: Basic Concepts & Preparation Procedures.

WARNING This product is not for human or veterinary use.

1. Li, Y., Bolinger, J., Yu, Y., et al. Intracellular delivery and biodistribution study of CRISPR/Cas9 ribonucleoprotein loaded bioreducible lipidoid nanoparticles. Biomater. Sci. 7(2), 596-606 (2019).

2. Liu, S., Cheng, Q., Wei, T., et al. Membrane-destabilizing ionizable phospholipids for organ-selective mRNA delivery and CRISPR-Cas gene editing. Nat. Mater. 20(5), 701-710 (2021).