Active • Host: HEK293 cells • AA: 33-449 • Tag: C-terminal His • MW: 46.5 kDa
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Axl Extracellular Domain (human, recombinant)

Item No. 44185

Product Insert (PDF)
Technical Information
Synonyms
  • UFO
Purity
≥98% as estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU per ug of the protein as determined by the LAL method
Source
Active recombinant human C-terminal His-tagged Axl extracellular domain expressed in HEK293 cells
Amino Acids
33-449
MW
46.5 kDa
Formulation (Request formulation change)
Lyophilized from sterile PBS, pH 7.4
UniProt Accession №
P30530
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $148

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    Product Description

    Axl is a receptor tyrosine kinase and member of the TAM kinase family.1,2 It is composed of two immunoglobulin-like (Ig-like) domains, two fibronectin-like III domains, an extracellular cleavage site, a transmembrane region, and a C-terminal kinase domain.1 It is ubiquitously expressed and localizes to the cell membrane.3,4 After homodimerization, Axl is activated by growth arrest-specific protein 6 (GAS6) and is involved in promoting proliferation and hematopoietic lineage differentiation and negatively regulating antigen-presenting cell activation.2,5,1 The extracellular domain of Axl can be cleaved by metalloproteinases, releasing soluble Axl to bind and activate membrane-associated Axl.4,3 In cancer, Axl promotes metastasis, epithelial-to-mesenchymal transition (EMT), and survival.1 Increased expression of AXL is associated with shorter progression-free survival in patients with non-small cell lung cancer (NSCLC).6 Cayman's Axl Extracellular Domain (human, recombinant) protein can be used for binding assays. This protein consists of 428 amino acids, has a calculated molecular weight of 46.5 kDa, and a predicted N-terminus of Glu33 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is 60-70 kDa due to glycosylation.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Graham, D.K., DeRyckere, D., Davies, K.D., et al. The TAM family: Phosphatidylserine sensing receptor tyrosine kinases gone awry in cancer. Nat. Rev. Cancer 14(12), 769-785 (2014).

    2. Axelrod, H., and Pienta, K.J. Axl as a mediator of cellular growth and survival. Oncotarget. 5(19), 8818-8852 (2014).

    3. Verma, A., Warner, S.L., Vankayalapati, H., et al. Targeting Axl and Mer kinases in cancer. Mol. Cancer Ther. 10(10), 1763-1773 (2016).

    4. Tondo, G., Perani, D., and Comi, C. TAM receptor pathways at the crossroads of neuroinflammation and neurodegeneration. Dis. Markers 2387614 (2019).

    5. Myers, K.V., Amend, S.R., and Pienta, K.J. Targeting Tyro3, Axl and MerTK (TAM receptors): Implications for macrophages in the tumor microenvironment. Mol. Cancer 18(1), 94 (2019).

    6. Yoshimura, A., Yamada, T., Serizawa, M., et al. High levels of AXL expression in untreated EGFR-mutated non-small cell lung cancer negatively impacts the use of osimertinib. Cancer Sci. 4(2), 606-618 (2023).