Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.
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Item No. 44311

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BSA-Eicosapentaenoate-d5 Polyunsaturated Fatty Acid Complex (1 mM) is composed of eicosapentaenoic acid-d5 (EPA-d5; Item No. 10005056) and fatty acid-free bovine serum albumin (BSA) at an approximately 6:1 molar ratio of eicosapentaenoate-d5:BSA. It is intended for the quantification of eicosapentaenoate-d5 uptake and incorporation by GC- or LC-MS. It can be used for efficient fatty acid delivery to cells in culture for the purpose of monitoring inflammatory signaling pathways, ferroptotic cell death, and lipid metabolism, including tracking the metabolic fate of EPA.1,2,3 Cayman’s BSA-Eicosapentaenoate-d5 Polyunsaturated Fatty Acid Complex (1 mM) is suitable for use in short-term cell culture applications (acute treatment to 18 hours); however, for long-term applications (25+ hours) the product should be filter-sterilized using a 0.2 µm filter and sterile receptacle, which will not affect its performance. For best results, it is recommended that this product be used in conjunction with Cayman’s BSA Control for BSA-fatty Acid Complexes (1 mM) (Item No. 34932), prepared with fatty acid-free BSA.
WARNING This product is not for human or veterinary use.
1. Cell culture models of fatty acid overload: Problems and solutions. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1863(2), 143-151 (2018).
2. LC-
3. Identification of essential sites of lipid peroxidation in ferroptosis. Nat. Chem. Biol. 19(6), 719-730 (2023).