A BSA complex with EPA
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

BSA-Eicosapentaenoate Polyunsaturated Fatty Acid Complex (1 mM)

Item No. 43473

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Bovine Serum Albumin-Eicosapentaenoate
  • Bovine Serum Albumin-EPA
  • BSA-EPA
  • BSA-Timnodonic Acid
Formulation (Request formulation change)
1 mM EPA:0.17 mM BSA in 150 mM sodium chloride
Origin
Animal/Bovine
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    5 ml

    $182

    Add
    Lipid Resource Center
    Discover Products & Resources for Lipid Research
    • High-purity lipid standards
    • Lipid roles in biology
    • Lipids in health & disease
    • Lipids for pharmaceutical development
    • Protocols, advice, & resources
    EXPLORE NOW
    Product Description

    BSA-Eicosapentaenoate Polyunsaturated Fatty Acid Complex (1 mM) is composed of eicosapentaenoic acid (EPA; Item No. 90110) and fatty acid-free bovine serum albumin (BSA) at an approximately 6:1 molar ratio of eicosapentaenoate:BSA. It can be used for efficient fatty acid delivery to cells in culture for the purpose of monitoring lipid metabolism and inflammatory signaling pathways.1,2 It can also be used to examine the effects of EPA on thermogenesis- and obesity-related gene expression and mitochondrial content, cancer cell growth, or glucose uptake in skeletal muscle cells.3,4,5 Cayman’s BSA-Eicosapentaenoate Polyunsaturated Fatty Acid Complex (1 mM) is suitable for use in short-term cell culture applications (acute treatment to 18 hours); however, for long-term applications (25+ hours) the product should be filter-sterilized using a 0.2 µm filter and sterile receptacle, which will not affect its performance. For best results, it is recommended that this product be used in conjunction with Cayman’s BSA Control for BSA-Fatty Acid Complexes (1 mM) (Item No. 34932), prepared with fatty acid-free BSA.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Alsabeeh, N., Chausse, B., Kakimoto, P.A., et al. Cell culture models of fatty acid overload: Problems and solutions. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1863(2), 143-151 (2018).

    2. Wiesenfeld, P.W., Babu, U.S., and O'Donnell, M.W. Effect of long-chain fatty acids in the culture medium on fatty acid composition of WEHI-3 and J774A.1 cells. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 128(1), 123-134 (2001).

    3. Pahlavani, M., Razafimanjato, F., Ramalingam, L., et al. Eicosapentaenoic acid regulates brown adipose tissue metabolism in high-fat-fed mice and in clonal brown adipocytes. J. Nutr. Biochem. 39, 101-109 (2017).

    4. Arita, K., Kobuchi, H., Utsumi, T., et al. Mechanism of apoptosis in HL-60 cells induced by n-3 and n-6 polyunsaturated fatty acids. Biochem. Pharmacol. 62(7), 821-828 (2001).

    5. Aas, V., Rokling-Andersen, M.H., Kase, E.T., et al. Eicosapentaenoic acid (20:5 n-3) increases fatty acid and glucose uptake in cultured human skeletal muscle cells. J. Lipid Res. 47(2), 366-374 (2006).