BSA-Polyunsaturated Fatty Acid Complex Mixture (0.33 mM)

BSA Polyunsaturated Fatty Acid Complex Mixture (0.33 mM) is composed of BSA-Docosahexaenoate Polyunsaturated Fatty Acid Complex (1 mM) (Item No. 35872), BSA-Arachidonate Polyunsaturated Fatty Acid Complex (1 mM) (Item No. 34931), and BSA-Eicosapentaenoate Polyunsaturated Fatty Acid Complex (1 mM) (Item No. 43473), which contain fatty acid-free bovine serum albumin (BSA) at an approximately 6:1 molar ratio of PUFA:BSA. This product enables the convenient delivery of all three PUFAs simultaneously. It can be used for efficient fatty acid delivery to cells in culture for the purpose of monitoring lipid metabolism and inflammatory signaling pathways.^1,2 Cayman’s BSA Polyunsaturated Fatty Acid Complex Mixture (0.33 mM) is suitable for use in short-term cell culture applications (acute treatment to 18 hours); however, for long-term applications (25+ hours) the product should be filter-sterilized using a 0.2 µm filter and sterile receptacle, which will not affect its performance. For best results, it is recommended that this product be used in conjunction with Cayman’s BSA Control for BSA-Fatty Acid Complexes (1 mM) (Item No. 34932), prepared with fatty acid-free BSA.

WARNING This product is not for human or veterinary use.

1. Alsabeeh, N., Chausse, B., Kakimoto, P.A., et al. Cell culture models of fatty acid overload: Problems and solutions. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1863(2), 143-151 (2018).

2. Wiesenfeld, P.W., Babu, U.S., and O'Donnell, M.W. Effect of long-chain fatty acids in the culture medium on fatty acid composition of WEHI-3 and J774A.1 cells. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 128(1), 123-134 (2001).