For immunoprecipitation of histone H3 (citrullinated R2 + R8 + R17)
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Citrullinated Histone H3 Immunoaffinity Resin

Item No. 44657

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Citrullinated Histone H3 Immunoaffinity Beads
  • Citrullinated Histone H3 Immunoaffinity Sorbent
Clone Designation
11D3
Formulation
200 µl of sepharose coupled to a histone H3 (Citrullinated R2 + R8 + R17) monoclonal antibody (Clone 11D3)
Storage Buffer
PBS, pH 7.2, with 0.02% sodium azide
Host
Mouse
Isotype
IgG1K
Applications
IP
Cross Reactivity
(+) Citrullinated histone H3
Species Reactivity
(+) Human
UniProt Accession №
P68431
Shipping & Storage Information
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

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    200 µl

    $299

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    Cayman Chemical
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    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications, including citrullination. Histone H3 is subject to citrullination of arginine residues by peptidyl arginine deiminase 4 (PAD4) at positions 2, 8, 17 and 26, and this citrullination blocks methylation of these residues by protein-arginine methyltransferase 4 (PRMT4) and induces transcriptional repression of target genes. Citrullinated histone H3 is a component of neutrophil extracellular traps (NETs) that acts as an autoantigen to induce production of anti-citrullinated protein antibodies associated with various diseases such as sepsis, multiple sclerosis, rheumatoid arthritis, and multiple myeloma.1,2,3,4 Cayman's Citrullinated Histone H3 Immunoaffinity Resin is designed for immunoprecipitation (IP) of citrullinated histone H3 from biological samples. This is an effective way to concentrate citrullinated histone H3 for subsequent detection and analysis by Western blot (WB). The citrullinated histone H3 immunoaffinity resin consists of Cayman's Histone H3 (Citrullinated R2 + R8 + R17) Monoclonal Antibody (Clone 11D3) (Item No. 17939) coupled to Sepharose.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Xu, Y.-M., Du, J.-Y., and Lau, A.T.Y. Posttranslational modifications of human histone H3: An update. Proteomics 14(17-18), 2047-2060 (2014).

    2. Li, Y., Liu, Z., Liu, B., et al. Citrullinated histone H3: A novel target for the treatment of sepsis. Surgery 156(2), 229-234 (2014).

    3. Moscarello, M.A., Mastronardi, F.G., and Wood, D.D. The role of citrullinated proteins suggests a novel mechanism in the pathogenesis of multiple sclerosis. Neurochem. Res. 32(2), 251-256 (2007).

    4. McNee, G., Eales, K.L., Wei, W., et al. Citrullination of histone H3 drives IL-6 production by bone marrow mesenchymal stem cells in MGUS and multiple myeloma. Leukemia 31(2), 373-381 (2017).