For immunochemical detection of citrullinated histone H3
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Histone H3 (Citrullinated R2 + R8 + R17) Monoclonal Antibody (Clone 11D3)

Item No. 17939

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Immunogen
Histone H3 peptide with citrulline at R2, R8, and R17
Clone Designation
11D3
MW
~16 kDa
Formulation (Request formulation change)
100 µg protein G-purified IgG
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Mouse
Isotype
IgG1K
Applications
ELISA, WB
Cross Reactivity
(-) Non-citrullinated histone H3
Species Reactivity
(+) Human; other species not tested
UniProt Accession №
P68431
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µg

    $440

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    Cayman Chemical
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    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications, including citrullination. Histone H3 is subject to citrullination of arginine residues by peptidyl arginine deiminase 4 (PAD4) at positions 2, 8, 17 and 26, and this citrullination blocks methylation of these residues by protein-arginine methyltransferase 4 (PRMT4) and induces transcriptional repression of target genes. Citrullinated histone H3 is a component of neutrophil extracellular traps (NETs) that acts as an autoantigen to induce production of anti-citrullinated protein antibodies associated with various diseases such as sepsis, multiple sclerosis, rheumatoid arthritis, and multiple myeloma.1,2,3,4 Cayman’s Histone H3 (Citrullinated R2 + R8 + R17) Monoclonal Antibody can be used for ELISA and Western blot applications. The antibody recognizes citrullinated histone H3 at approximately 16 kDa from human samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Xu, Y.-M., Du, J.-Y., and Lau, A.T.Y. Posttranslational modifications of human histone H3: An update. Proteomics 14(17-18), 2047-2060 (2014).

    2. Li, Y., Liu, Z., Liu, B., et al. Citrullinated histone H3: A novel target for the treatment of sepsis. Surgery 156(2), 229-234 (2014).

    3. Moscarello, M.A., Mastronardi, F.G., and Wood, D.D. The role of citrullinated proteins suggests a novel mechanism in the pathogenesis of multiple sclerosis. Neurochem. Res. 32(2), 251-256 (2007).

    4. McNee, G., Eales, K.L., Wei, W., et al. Citrullination of histone H3 drives IL-6 production by bone marrow mesenchymal stem cells in MGUS and multiple myeloma. Leukemia 31(2), 373-381 (2017).

    Product Citations

    Thålin, C., Aguilera, K., Hall, N.W., et al. Quantification of citrullinated histones: Development of an improved assay to reliably quantify nucleosomal H3Cit in human plasma. J. Thromb. Haemost. 18(10), 2732-2743 (2020).