A BSA-fatty acid complex and paired control for use in cell culture
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BSA-Linoleate Reagent Set (1 mM)

Item No. 44717

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Bovine Serum Albumin-LA
  • Bovine Serum Albumin-Linoleate
  • BSA-LA
  • BSA-9(Z),12(Z)-Octadecadienoic Acid
Source
Bovine
Formulation (Request formulation change)
Item No. 44718: 1 mM linoleate:0.17 mM BSA in 150 mM sodium chloride, pH 7.4; Item No. 44719: 0.17 mM BSA in 150 mM sodium chloride, pH 7.4
Origin
Animal/Bovine
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea

    $196

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    Product Description

    The BSA-Linoleate Reagent Set (1 mM) contains a BSA-linoleate complex solution and a fatty acid-free BSA control solution. BSA-Linoleate is composed of linoleic acid (Item No. 90150) and fatty acid-free bovine serum albumin (BSA) at an approximately 6:1 molar ratio of linoleate:BSA. The BSA-linoleate complex and BSA control were prepared at the same time with the same lot of fatty acid-free BSA under sterile conditions, then filtered and aliquoted into sterile vials. The BSA-Linoleate Reagent Set (1 mM) can be used for efficient fatty acid delivery to cells in culture for the purpose of monitoring lipid metabolism and inflammatory signaling pathways.1,2,3,4,5 Cayman's BSA-Linoleate Reagent Set (1 mM) is suitable for use in short- and long-term cell culture applications (25+ hours).

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Alsabeeh, N., Chausse, B., Kakimoto, P.A., et al. Cell culture models of fatty acid overload: Problems and solutions. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1863(2), 143-151 (2018).

    2. Menon, D., Salloum, D., Bernfeld, E., et al. Lipid sensing by mTOR complexes via de novo synthesis of phosphatidic acid. The Journal of Biological Chemisty 292(15), 6303-6311 (2017).

    3. Wiesenfeld, P.W., Babu, U.S., and O'Donnell, M.W. Effect of long-chain fatty acids in the culture medium on fatty acid composition of WEHI-3 and J774A.1 cells. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 128(1), 123-134 (2001).

    4. Colquhoun, A., and Curi, R. Regulation of tumour cell fatty acid oxidation by n-6 polyunsaturated fatty acids. Biochem. Soc. Trans. 25(4), S681 (1997).

    5. Mukherjee, A., Adhikari, A., Das, P., et al. Loss of virulence in NAD(P)H cytochrome b5 oxidoreductase deficient Leishmania major. Biochem. Biophys. Res. Commun. 503(1), 371-377 (2018).