AGE-BSA Reagent Set (Glucose modified)

Advanced glycation end products (AGEs) are formed from the non-enzymatic reaction of amino groups with reducing sugars.^1,2,3 AGEs have been implicated in several diseases, such as diabetes mellitus, non-diabetic nephropathy, macrovascular disease, Alzheimer’s disease, cataracts, and aging. AGE receptors, such as the receptor for AGEs (RAGE), mediate biological responses to AGEs, including endocytic uptake and degradation and induction of cytokines and growth factors. AGE-BSA has been used as a standard for AGEs in ELISAs and to determine the effects of AGEs on cells in culture.^4,5,6 AGE-BSA was produced by incubating BSA with glucose, followed by extensive dialysis. The AGE-BSA and BSA control were prepared at the same time with the same lot of BSA under sterile conditions, then filtered and aliquoted into sterile vials and lyophilized.

WARNING This product is not for human or veterinary use.

1. Singh, R., Barden, A., Mori, T., et al. Advanced glycation end-products: A review. Diabetologia 44(2), 129-146 (2001).

2. Giacco, F., and Brownlee, M. Oxidative stress and diabetic complications. Circ. Res. 107(9), 1058-1070 (2010).

3. Vistoli, G., De Maddis, D., Cipak, A., et al. Advanced glycoxidation and lipoxidation end products (AGEs and ALEs): An overview of their mechanisms of formation. Free Radic. Res. 47(Suppl 1), 3-27 (2013).

4. Cai, W., Gao, Q.d., Zhu, L., et al. Oxidative stress-inducing carbonyl compounds from common foods: Novel mediators of cellular dysfunction. Mol. Med. 8(7), 337-346 (2002).

5. Dedert, C., Mishra, V., Aggarwal, G., et al. Progranulin preserves autophagy flux and mitochondrial function in rat cortical neurons under high glucose stress. Front. Cell. Neurosci. 16, 874258 (2022).

6. Xia, C., Zhang, J., Chen, H., et al. ShenQi ShenKang granule alleviates chronic kidney disease by inhibiting the PI3K/AKT/mTOR pathway and restoring autophagy flux and mitochondrial integrity. Drug Des. Devel. Ther. 19, 3925-3947 (2025).