BSA-Docosahexaenoate Polyunsaturated Fatty Acid Complex (5 mM)

BSA-Docosahexaenoate Polyunsaturated Fatty Acid Complex (5 mM) is composed of docosahexaenoic acid (DHA; Item No. 90310) and fatty acid-free bovine serum albumin (BSA) at an approximately 6:1 molar ratio of docosahexaenoate:BSA. It can be used for efficient fatty acid delivery to cells in culture for the purpose of monitoring lipid metabolism and inflammatory signaling pathways.^1,2 BSA-DHA complexes have been used in proliferation, migration, and cell death assays to study the role of DHA in cancer in vitro.³ Cayman’s BSA-Docosahexaenoate Polyunsaturated Fatty Acid Complex (5 mM) is suitable for use in short-term cell culture applications (acute treatment to 18 hours); however, for long-term applications (25+ hours) the product should be filter-sterilized using a 0.2 µm filter and sterile receptacle, which will not affect its performance. This product can be used to achieve higher concentrations or perform larger experiments than can be achieved with BSA-Docosahexaenoate Polyunsaturated Fatty Acid Complex (1 mM) (Item No. 35872). For best results, it is recommended that this product be used in conjunction with Cayman’s BSA Control for BSA-Fatty Acid Complexes (5 mM) (Item No. 29556), prepared with fatty acid-free BSA.

WARNING This product is not for human or veterinary use.

1. Alsabeeh, N., Chausse, B., Kakimoto, P.A., et al. Cell culture models of fatty acid overload: Problems and solutions. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1863(2), 143-151 (2018).

2. Katsnelson, G., and Ceddia, R.B. Docosahexaenoic and eicosapentaenoic fatty acids differentially regulate glucose and fatty acid metabolism in L6 rat skeletal muscle cells. Am. J. Physiol. Cell Physiol. 319(6), C1120-C1129 (2020).

3. Sun, S.N., Jia, W.D., Chen, H., et al. Docosahexaenoic acid (DHA) induces apoptosis in human hepatocellular carcinoma cells. Int. J. Clin. Exp. Pathol. 6(2), 281-289 (2013).

Low, Y.L., Pan, Y., Short, J.L., et al. Profiling the expression of fatty acid-binding proteins and fatty acid transporters in mouse microglia and assessing their role in docosahexaenoic acid-d₅ uptake. Prostaglandins Leukot. Essent. Fatty Acids 171:102303, (2021).