An SM-102-based LNP solution for expressing human cGAS
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

LipidLaunch™ SM-102 LNP (human cGAS)

Item No. 44930

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formulation (Request formulation change)
TBS, pH 7.5, with 10% sucrose
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    5 µg

    $499

    Add
    Cayman Chemical
    Product Description

    LipidLaunch™ SM-102 LNP (human cGAS) is a solution containing lipid nanoparticles (LNPs) composed of the ionizable cationic aminolipid SM-102 (Item No. 33474), cholesterol (Item No. 9003100), 1,2-distearoyl-sn-glycero-3-PC (1,2-DSPC; Item No. 15100), and the lipid excipient DMG-PEG(2000) (Item No. 33945) at a molar ratio of 50:38.5:10:1.5 and encapsulating mRNA encoding His-tagged human cyclic GMP-AMP (cGAMP) synthase (cGAS), a nucleotidyltransferase involved in the innate immune response.1,2 It is intended for the overexpression of His-tagged human wild-type cGAS in vitro and can be used in a variety of model cell lines.

    Suggested in vitro use: Thaw LNPs on ice with occasional gentle swirling (do not vortex). Using a gentle pipetting technique, dilute 1:100-1:500 in complete cell culture media (with serum) and add to subconfluent cells. Expression of His-tagged human cGAS may be detected between six and 48 hours after transfection. Optimal conditions are highly dependent on cell type.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Sun, L., Wu, J., Du, F., et al. Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway. Science 339(6121), 786-791 (2013).

    2. Wu, J., Sun, L., Chen, X., et al. Cyclic GMP-AMP is an endogenous second messenger in innate immune signaling by cytosolic DNA. Science 339(6121), 826-830 (2013).