BSA-Myristate Saturated Fatty Acid Complex (5 mM)

BSA-Myristate Saturated Fatty Acid Complex (5 mM) is composed of myristic acid (Item No. 13351) and fatty acid-free bovine serum albumin (BSA) at an approximately 6:1 molar ratio of myristate:BSA. It can be used for efficient fatty acid delivery to cells in culture for the purpose of monitoring lipid metabolism and inflammatory signaling pathways and studying the role of myristic acid in the induction of endoplasmic reticulum (ER) stress.^1,2 Cayman’s BSA-Myristate Saturated Fatty Acid Complex (5 mM) is suitable for use in short-term cell culture applications (acute treatment to 18 hours); however, for long-term applications (25+ hours) the product should be filter-sterilized using a 0.2 µm filter and sterile receptacle, which will not affect its performance. For best results, it is recommended that this product be used in conjunction with Cayman’s BSA Control for BSA-Fatty Acid Complexes (5 mM) (Item No. 29556), prepared with fatty acid-free BSA.

WARNING This product is not for human or veterinary use.

1. Alsabeeh, N., Chausse, B., Kakimoto, P.A., et al. Cell culture models of fatty acid overload: Problems and solutions. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1863(2), 143-151 (2018).

2. Choi, S., Snider, J.M., Olakkengil, N., et al. Myristate-induced endoplasmic reticulum stress requires ceramide synthases 5/6 and generation of C₁₄-ceramide in intestinal epithelial cells. The FASEB Journal 32(10), 5724-5736 (2018).