A chemiluminescent probe
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ABEI

Item No. 45127

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
7-[(4-aminobutyl)ethylamino]-4-hydroxy-1(2H)-phthalazinone
CAS Number
66612-29-1
Molecular Formula
C14H20N4O2
Formula Weight
276.3
Purity
≥98%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Acetonitrile:Water (1:1): Slightly soluble: 0.1-1 mg/ml
SMILES
O=C1NNC(C2=CC(N(CCCCN)CC)=CC=C12)=O
InChi Code
InChI=1S/C14H20N4O2/c1-2-18(8-4-3-7-15)10-5-6-11-12(9-10)14(20)17-16-13(11)19/h5-6,9H,2-4,7-8,15H2,1H3,(H,16,19)(H,17,20)
InChi Key
LEOJISUPFSWNMA-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    50 mg

    $135

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    100 mg

    $203

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    250 mg

    $473

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    500 mg

    $878

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    Product Description

    ABEI is a chemiluminescent probe and a derivative of isoluminol (Item No. 45126).1 It has been used for the detection of reactive oxygen species (ROS).2 ABEI reacts with amine-, thiol-, and carboxylic acid-moieties to form conjugates with small molecules for their detection in HPLC applications.3,4 It also has been conjugated to antibodies for use in immunoassays.5 ABEI displays a chemiluminescent emission maximum of 425 nm.6

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Schroeder, H.R., and Yeager, F.M. Chemiluminescence yields and detection limits of some isoluminol derivatives in various oxidation systems. Anal. Chem. 50(8), 1114-1120 (1978).

    2. Hosaka, S., Itagaki, T., and Kuramitsu, Y. Selectivity and sensitivity in the measurement of reactive oxygen species (ROS) using chemiluminescent microspheres prepared by the binding of acridinium ester of ABEI to polymer microspheres. Luminescence 14(6), 349-354 (1999).

    3. Li, C., Zhu, M., Liu, S., et al. Development of nitric oxide-donating netarsudil derivatives as a synergistic therapy for glaucoma with reduced ocular irritation. J. Med. Chem. 67(18), 16311-16327 (2024).

    4. Zhang, Y., Chai, Y., Wang, H., et al. Target-induced 3D DNA network structure as a novel signal amplifier for ultrasensitive electrochemiluminescence detection of microRNAs. Anal. Chem. 91(22), 14368-14374 (1998).

    5. Messeri, G., Orlandini, A., and Pazzagli, M. Luminescent immunoassay using isoluminol derivatives. J. Biolumin. Chemilumin. 4(1), 154-158 (1989).

    6. Huang, X., Deng, T., Wang, Q., et al. Highly emissive luminophores for direct chemiluminescence detection. Org. Lett. 27(23), 5942-5947 (2025).