Active • Host: Insect cells • AA: 2-474 (full length) • MW: 52.4 kDa
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Cbl-c (human, recombinant)

Item No. 45177

Product Insert (PDF)
Technical Information
Synonyms
  • Casitas B-lineage Lymphoma Proto-oncogene C
  • CBLC
  • Cbl-3
  • Cbl Proto-oncogene C
  • Cbl-SL
  • RNF57
  • RING Finger Protein 57
  • E3 Ubiquitin-protein Ligase Cbl-c
Purity
≥90% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/µg, determined by the LAL endotoxin assay
Source
Active recombinant human Cbl-c expressed in insect cells
Amino Acids
2-474
MW
52.4 kDa
Formulation (Request formulation change)
50 mM Tris-HCl, pH 7.5, with 200 mM sodium chloride and 20% glycerol
Host
Insect cells
UniProt Accession №
Q9ULV8
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $1,178

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    500 µg

    $4,139

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    Product Description

    Casitas B-lineage lymphoma proto-oncogene C (Cbl-c) is a member of the Cbl RING-type E3 ubiquitin ligase family.1 It is composed of an N-terminal tyrosine kinase-binding domain, which contains a helix bundle, an EF hand calcium-binding motif, and a variant Src homology 2 (SH2) domain, a linker region, a RING finger domain, and a proline-rich region.2,3 Cbl-c is autoinhibited by its EF hand and SH2 domain but is activated following Tyr341 phosphorylation by Src.2 It is expressed in colon, small intestine, pancreas, placenta, liver, kidney, and prostate.3 Cbl-c is involved in the negative regulation of receptor tyrosine kinases, including EGFR and Fyn.1,3 Intratumoral levels of Cbl-c are increased in patients with colorectal cancer and loss-of-function mutations in CBLC are associated with several cancers.4,5 Cayman’s Cbl-c (human, recombinant) protein can be used for enzyme activity assays, structural biology, and biophysical applications and has a calculated molecular weight of 52.4 kDa.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Tang, R., Langdon, W.Y., and Zhang, J. Negative regulation of receptor tyrosine kinases by ubiquitination: Key roles of the Cbl family of E3 ubiquitin ligases. Front. Endocrinol. (Lausanne) 13, 971162 (2022).

    2. Ryan, P.E., Sivadasan-Nair, N., Nau, M.M., et al. The N terminus of Cbl-c regulates ubiquitin ligase activity by modulating affinity for the ubiquitin-conjugating enzyme. The Journal of Biological Chemisty 285(31), 23687-23698 (2010).

    3. Kim, M., Tezuka, T., Suziki, Y., et al. Molecular cloning and characterization of a novel cbl-family gene, cbl-c. Gene 239(1), 145-154 (1999).

    4. Li, Z., Yan, G., Yang, M., et al. CBLC promotes the development of colorectal cancer by promoting ABI1 degradation to activate the ERK signaling pathway. Transl. Oncol. 45, 101992 (2024).

    5. Daniels, S.R., Liyasova, M., Kales, S.C., et al. Loss of function Cbl-c mutations in solid tumors. PLoS One 14(7), e0219143 (2019).