Active • Host: Insect cells • AA: 1-442 (CRBN) (full length), 1-1,140 (DDB1) (full length) • Tag: N-terminal His-AVI on CRBN • MW: 55.3 (CRBN) and 126.9 (DDB1) kDa
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CRBN/DDB1 Complex (human, recombinant)

Item No. 45180

Product Insert (PDF)
Technical Information
Synonyms
  • Cereblon
  • Damage-specific DNA-binding Protein 1
  • DNA Damage-binding Protein 1
  • HBV X-associated Protein 1
  • UV-damaged DNA-binding Factor
  • UV-damaged DNA-binding Protein 1
  • UV-DDB1
  • XAP-1
  • Xeroderma Pigmentosum Group E-complementing Protein
  • XPE-BF
  • XPE-binding Factor
Purity
≥90% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/μg, determined by the LAL endotoxin assay
Source
Active recombinant human N-terminal His-AVI-tagged CRBN and DDB1 expressed in insect cells
Amino Acids
1-442 (CRBN) (full length), 1-1,140 (DDB1) (full length)
MW
55.3 (CRBN) and 126.9 (DDB1) kDa
Formulation (Request formulation change)
50 mM Tris-HCl, pH 7.5, with 200 mM sodium chloride, 20% glycerol, and 1 mM DTT
UniProt Accession №
Q96SW2/Q16531
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $932

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    500 µg

    $3,218

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    Cayman Chemical
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    Product Description

    The cullin 4 RING ligase (CRL4) complex is an E3 ubiquitin ligase complex.1,2,3 It is composed of the substrate receptor cereblon (CRBN), DNA damage-binding protein 1 (DDB1), cullin 4A or 4B, and RING box protein 1 (RBX1). CRBN interacts with DDB1 via its helical bundle between the Lon protease-like domain and thalidomide-binding domain. The CRL4 complex targets substrates for proteasomal degradation by ubiquitination and is involved in regulation of the cell cycle, embryogenesis, DNA repair, replication, transcription, and carcinogenesis.2 Mutations in CRBN are associated with autosomal recessive non-syndromic intellectual disability, and CRBN is also the target of thalidomide-induced teratogenicity.2,3,4 Mutations in DDB1 are also associated with intellectual disability, as well as obesity, digital anomalies, hypotonia, and malformations.5 Cayman’s CRBN/DDB1 Complex (human, recombinant) protein can be used for enzyme activity assay and binding assay applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Petroski, M.D., and Deshaies, R.J. Function and regulation of cullin-RING ubiquitin ligases. Nat. Rev. Mol. Cell Biol. 6(1), 9-20 (2005).

    2. Kim, H.K., Ko, T.H., Nyamaa, B., et al. Cereblon in health and disease. Pflugers Arch. 468(8), 1299-1309 (2016).

    3. Kim, H.K., Seol, J.E., Ahn, S.W., et al. Cereblon: Promise and challenges for combating human diseases. Pflugers Arch. 473(11), 1695-1711 (2021).

    4. Ito, T., Ando, H., Suzuki, T., et al. Identification of a primary target of thalidomide teratogenicity. Science 327(5971), 1345-1350 (2010).

    5. White, S.M., Bhoj, E., Nellåker, C., et al. A DNA repair disorder caused by de novo monoallelic DDB1 variants is associated with a neurodevelopmental syndrome. Am. J. Hum. Genet. 108(4), 749-756 (2021).