Active • Host: Insect cells • AA: 1-442 (CRBN) (full length), 1-1,140 (DDB1) (full length), 2-759 (cullin 4A) (full length), 1-108 (RBX1) (full length) • Tag: N-terminal His-AVI • MW: 55.3 (CRBN), 126.9 (DDB1), 87.6 (cullin 4A), and 12.2 (RBX1) kDa
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

CRBN/DDB1/Cullin 4A/RBX1 Complex (human, recombinant)

Item No. 45181

Product Insert (PDF)
Technical Information
Synonyms
  • CRBN: Cereblon
  • Cullin 4A: CUL4A
  • DDB1: Damage-specific DNA-binding Protein 1
  • DDB1: DNA Damage-binding Protein 1
  • DDB1: HBV X-associated Protein 1
  • DDB1: UV-damaged DNA-binding Factor
  • DDB1: UV-damaged DNA-binding Protein 1
  • DDB1: UV-DDB1
  • DDB1: XAP-1
  • DDB1: Xeroderma Pigmentosum Group E-complementing Protein
  • DDB1: XPE-BF
  • DDB1: XPE-binding Factor
  • RBX1: Regulator of Cullins-1
  • RBX1: RING Box Protein 1
  • RBX1: RING Finger Protein 75
  • RBX1: RNF75
  • RBX1: ROC1
Purity
≥90%
Endotoxin Testing
<1 EU per µg of the protein
Source
Active recombinant human N-terminal His-AVI-tagged CRBN, DDB1, cullin 4A, and RBX1 expressed in insect cells
Amino Acids
1-442 (CRBN) (full length), 1-1,140 (DDB1) (full length), 2-759 (cullin 4A) (full length), 1-108 (RBX1) (full length)
MW
55.3 (CRBN), 126.9 (DDB1), 87.6 (cullin 4A), and 12.2 (RBX1) kDa
Formulation (Request formulation change)
50 mM Tris-HCl, pH 7.5, with 200 mM sodium chloride, 20% glycerol, and 1 mM DTT
UniProt Accession №
Q96SW2/Q16531/Q13619/P62877
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    50 µg

    $975

    Add
    Cayman Chemical
    Explore Obesity & Metabolic Disease Resources

    Discover high-quality research tools to investigate GLP-1 mechanisms and next-generation metabolic targets.

    OBESITY RESEARCH SOLUTIONS
    Product Description

    The cullin 4 RING ligase (CRL4) complex is an E3 ubiquitin ligase complex.1,2,3 It is composed of the substrate receptor cereblon (CRBN), DNA damage-binding protein 1 (DDB1), cullin 4A or 4B, and RING box protein 1 (RBX1). CRBN and RBX1 bind to DDB1 and cullin 4A, respectively, and cullin 4A bridges DDB1 and RBX1, a configuration that allows CRBN to bind CRL4 substrates in proximity to RBX1 for ubiquitination.2 In this way, the CRL4 complex targets substrates for proteasomal degradation and is involved in regulation of the cell cycle, embryogenesis, DNA repair, replication, transcription, and carcinogenesis. Mutations in CRBN are associated with autosomal recessive non-syndromic intellectual disability, and CRBN is also the target of thalidomide-induced teratogenicity.2,3,4 Mutations in DDB1 are also associated with intellectual disability, as well as obesity, digital anomalies, hypotonia, and malformations.5 RBX1 and CUL4A, the gene encoding cullin 4A, are separately overexpressed in a variety of cancers and associated with poor overall survival.6,7,8 Cayman’s CRBN/DDB1/Cullin 4A/RBX1 Complex (human, recombinant) can be used for enzyme activity assays.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Petroski, M.D., and Deshaies, R.J. Function and regulation of cullin-RING ubiquitin ligases. Nat. Rev. Mol. Cell Biol. 6(1), 9-20 (2005).

    2. Kim, H.K., Ko, T.H., Nyamaa, B., et al. Cereblon in health and disease. Pflugers Arch. 468(8), 1299-1309 (2016).

    3. Kim, H.K., Seol, J.E., Ahn, S.W., et al. Cereblon: Promise and challenges for combating human diseases. Pflugers Arch. 473(11), 1695-1711 (2021).

    4. Ito, T., Ando, H., Suzuki, T., et al. Identification of a primary target of thalidomide teratogenicity. Science 327(5971), 1345-1350 (2010).

    5. White, S.M., Bhoj, E., Nellåker, C., et al. A DNA repair disorder caused by de novo monoallelic DDB1 variants is associated with a neurodevelopmental syndrome. Am. J. Hum. Genet. 108(4), 749-756 (2021).

    6. Lee, J., and Zhou, P. Pathogenic role of the CRL4 ubiquitin ligase in human disease. Front. Oncol. 2:21, (2012).

    7. Wang, W., Qiu, J., Liu, Z., et al. Overexpression of RING box protein-1 (RBX1) associated with poor prognosis of non-muscle-invasive bladder transitional cell carcinoma. J. Surg. Oncol. 107(7), 758-761 (2013).

    8. Xing, R., Chen, K.-B., Xuan, Y., et al. RBX1 expression is an unfavorable prognostic factor in patients with non-small cell lung cancer. Surg. Oncol. 25(3), 147-151 (2016).