A fluorescently tagged phosphatidylethanolamine
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NBD-PE

Item No. 45712

Technical Information
Formal Name
hexadecanoic acid, 1,1′-[[(1R)-1-[[[hydroxy[2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]ethoxy]phosphinyl]oxy]methyl]-1,2-ethanediyl] ester, compd. with N,N-diethylethanamine (1:1)
CAS Number
178119-00-1
Synonyms
  • NBD-1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine
  • NBD-PtdEtn
Molecular Formula
C43H75N4O11P • C6H15N
Formula Weight
Purity
≥95%
Emission
~525 nm
Excitation
~460 nm
A solid
Methanol: Slightly soluble: 0.1-1 mg/ml
SMILES
CCCCCCCCCCCCCCCC(O[C@@H](COP(OCCNC1=CC=C(C2=NON=C21)[N+]([O-])=O)(O)=O)COC(CCCCCCCCCCCCCCC)=O)=O.CCN(CC)CC
InChi Code
InChI=1S/C43H75N4O11P.C6H15N/c1-3-5-7-9-11-13-15-17-19-21-23-25-27-29-40(48)54-35-37(57-41(49)30-28-26-24-22-20-18-16-14-12-10-8-6-4-2)36-56-59(52,53)55-34-33-44-38-31-32-39(47(50)51)43-42(38)45-58-46-43;1-4-7(5-2)6-3/h31-32,37,44H,3-30,33-36H2,1-2H3,(H,52,53);4-6H2,1-3H3/t37-;/m1./s1
InChi Key
LEJHDNAGUHKMLR-GKEJWYBXSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    Product Description

    NBD-PE is a fluorescently tagged phosphatidylethanolamine containing palmitic acid (Item Nos. 10006627 | 43561) at the sn-1 and sn-2 positions. NBD-PE displays excitation/emission maxima of ~460/525 nm, respectively, in ethanol.1 It has been incorporated into plasma and mitochondrial membranes and liposomes, used to study lipid rafts, and used as a substrate in phospholipidosis assays.1,2,3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Monti, J.A., Christian, S.T., and Shaw, W.A. Synthesis and properties of a highly fluorescent derivative of phosphatidylethanolamine. J. Lipid Res. 19(2), 222-228 (1978).

    2. de Almeida, R.F.M., Loura, L.M.S., Fedorov, A., et alLipid rafts have different sizes depending on membrane composition: A time-resolved fluorescence resonance energy transfer study. J. Mol. Biol. 346(4), 1109-1120 (2005).

    3. Bhandari, N., Figueroa, D.J., Lawrence, J.W., et alPhospholipidosis assay in HepG2 cells and rat or rhesus hepatocytes using phospholipid probe NBD-PE. Assay Drug. Dev. Technol. 6(3), 407-419 (2008).