Host: HEK293 cells • AA: 22-124 • MW: ~11 kD (monomer); ~53 kD (pentamer)
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Unlabeled Version(s)
46349Cholera Toxin B Subunit (recombinant)
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Cholera Toxin B Subunit (recombinant) - 488

Item No. 46157

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Cholera Enterotoxin B Chain
  • Cholera Enterotoxin Gamma Chain
  • Cholera Enterotoxin Subunit B
  • Choleragenoid
  • CTB
  • CTxB
Purity
≥90%
Emission
513 nm
Excitation
492 nm
Source
Recombinant CTB expressed in HEK293 cells
Amino Acids
22-124
MW
~11 kD (monomer); ~53 kD (pentamer)
Formulation (Request formulation change)
1 mg/ml after resuspension in 100 µl of water
UniProt Accession №
P01556
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µg

    $440

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    Product Description

    Cholera Toxin B Subunit (recombinant) - 488 is a fluorescently labeled form of cholera toxin B subunit, a multimeric endotoxin produced by V. cholerae.1,2 The non-toxic pentameric B subunit of cholera toxin binds to ganglioside GM1 (Item No. 19579), which acts as a receptor on mammalian cells, and facilitates entrance of the toxic A subunit into cells.3,1 Cholera toxin B subunit (CTB) has been widely used in glycosphingolipid and membrane biology to study lipid rafts, nanodomain assembly, membrane organization and curvature, clathrin-independent endocytosis, and retrograde trafficking in the central nervous system (CNS) either alone or conjugated to a reporter.4,5,6 It has also been used as a carrier protein and an adjuvant, where it increases IgA immune responses, antigen presentation, B cell differentiation, and T cell proliferation.7,8 Cayman’s Cholera Toxin B Subunit (recombinant) - 488 can be used in cell culture at 1-4 µg/ml and for ELISA and GM1 binding assay applications. It displays excitation/emission maxima of 492/513 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Cho, J.A., Chinnapen, D.J.F., Amar, E., et al. Insights on the trafficking and retro-translocation of glycosphingolipid-binding bacterial toxins. Front. Cell. Infect. Microbiol. 2(51), (2012).

    2. Baldauf, K.J., Royal, J.M., Hamorsky, K.T., et al. Cholera toxin B: One subunit with many pharmaceutical applications. Toxins (Basel) 7(3), 974-996 (2015).

    3. Turnbull, W.B., Precious, B.L., and Homans, S.W. Dissecting the cholera toxin-ganglioside GM1 interaction by isothermal titration calorimetry. J. Am. Chem. Soc. 126(4), 1047-1054 (2004).

    4. Blank, N., Schiller, M., Krienke, S., et al. Cholera toxin binds to lipid rafts but has a limited specificity for ganglioside GM1. Immunol. Cell Biol. 85(5), 378-382 (2007).

    5. Kenworthy, A.K., Schmieder, S.S., Raghunathan, K., et al. Cholera toxin as a probe for membrane biology. Toxins (Basel) 13(8), 543 (2021).

    6. Luppi, P.-H., Fort, P., and Jouvet, M. Iontophoretic application of unconjugated cholera toxin B subunit (CTb) combined with immunohistochemistry of neurochemical substances: A method for transmitter identification of retrogradely labeled neurons. Brain Res. 534(1-2), 209-224 (1990).

    7. Siriwardhana, C., Bajracharya, A., Seal, F., et al. Evaluation of cholera toxin B subunit as a novel carrier protein for polysaccharide conjugate vaccines. Vaccines (Basel) 13(11), 1159 (2025).

    8. Holmgren, J., Lycke, N., and Czerkinsky, C. Cholera toxin and cholera B subunit as oral-mucosal adjuvant and antigen vector systems. Vaccine 11(12), 1179-1184 (1993).