Features
- Convenient 96-well plate format
- Measure Grx activity in cell lysates and tissue samples
- Active Grx provided as a positive control
- Eosin-labelled glutathione is used as a substrate – excitation at 520 nm and emission at 560 nm
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OBESITY RESEARCH SOLUTIONSProduct Description
Cayman’s Glutaredoxin Fluorometric Activity Assay Kit provides a convenient method of measuring Grx activity in cell lysates and tissue samples. In this reaction, the substrate eosin-GS-BSA (self-quenched) is reduced by Grx, giving the fluorescent product eosin-GSH that can be detected at an emission wavelength of 560 nm. Grx becomes oxidized during the reaction and then reduced by glutathione to produce GSSG, which is recycled by a coupled reaction via glutathione reductase and NADPH (see Reaction Schematic image). The increase in signal is directly proportional to the Grx activity in the sample.
Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.
WARNING This product is not for human or veterinary use.
Contact Info
Cayman Chemical1180 East Ellsworth RoadAnn Arbor, Michigan 48108 USAToll Free: (800) 364-9897(USA and Canada only)Fax: (734) 971-3640
