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Citrullinated Histone H3 (Clone 11D3) ELISA Kit

Item № 501620
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 wells $495.00 0.00

Pricing updated 2019-07-17. Prices are subject to change without notice.

Description
Features
  • Detect CitH3 in cell culture supernatants, cell lysates, human plasma, and human serum
  • Detect CitH3 from as few as 1,000 neutrophils in human and mouse
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure CitH3 levels down to 0.15 ng/ml
  • Rapid assay; get results in under 4 hours
Synonyms
  • CitH3 (Clone 11D3) EIA Kit

Histone H3 is citrullinated by peptidylarginine deiminase 4 (PAD4) at residues R2, R8, and R17 during the processes of epigenetic chromatin remodeling and neutrophil extracellular trap (NET) formation. In vitro, citrullinated H3 (CitH3) is released from neutrophils that have been stimulated with calcium ionophore during the process of NETosis, and can be freed from NETs by treatment with nuclease. The measurement of CitH3, either in cell lysates or released during NETosis can be used as a functional readout of pharmacological PAD inhibition.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • CitH3 (Clone 11D3) EIA Kit
Clone Designation
11D3
Limit of Detection
0.1 ng/ml
Assay Range
0.15-10 ng/ml
Lower Limit of Quantification
0.3 ng/ml
Origin
  • Animal/Bovine
  • Animal/Mouse

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Citations

Murthy, P., Singhi, A.D., Ross, M.A., et al. Enhanced neutrophil extracellular trap formation in acute pancreatitis contributes to disease severity and is reduced by chloroquine Front. Immunol. 10, 28 (2019).

Technical Support
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