An ELISA for quantifying Citrullinated Histone H3 in biological matrices
Features
  • Detect CitH3 in cell culture supernatants, cell lysates, human plasma, and human serum
  • Detect CitH3 from as few as 1,000 neutrophils in human and mouse
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure CitH3 levels down to 0.15 ng/ml
  • Rapid assay; get results in under 4 hours
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Citrullinated Histone H3 (Clone 11D3) ELISA Kit

Item No. 501620

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • CitH3 (Clone 11D3) EIA Kit
Clone Designation
11D3
Limit of Detection
0.1 ng/ml
Assay Range
0.15-10 ng/ml
Lower Limit of Quantification
0.3 ng/ml
Origin
Animal/Bovine, Animal/Mouse
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $575

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    NET Formation Screening & Analysis Services
    Our experts are here to help.
    • Detection of NET formation ex vivo and screening for modulators using:
      • High-content imaging
      • Enzymatic detection
      • Citrullination/carbamylation detection
    • Experienced scientists skilled in neutrophil biology, isolation, and handling
    LEARN MORE
    Product Description

    Histone H3 is citrullinated by peptidylarginine deiminase 4 (PAD4) at residues R2, R8, and R17 during the processes of epigenetic chromatin remodeling and neutrophil extracellular trap (NET) formation. In vitro, citrullinated H3 (CitH3) is released from neutrophils that have been stimulated with calcium ionophore during the process of NETosis, and can be freed from NETs by treatment with nuclease. The measurement of CitH3, either in cell lysates or released during NETosis can be used as a functional readout of pharmacological PAD inhibition.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Barakzie, A., van der Steen, W., Jansen, A.J.G., et al. Association of NETs markers with clinical and radiological outcomes in patients with acute ischemic stroke undergoing thrombectomy: Does heparin treatment modify this? Transl. Stroke Res. 16, 1985-1995 (2025).

    Melamud, M.M., Tolmacheva, A.S., Sizikov, A.E., et al. NETosis-related biomarkers in systemic lupus erythematosus, rheumatoid arthritis, psoriatic arthritis and ankylosing spondylitis: A comparative study. Int. J. Mol. Sci. 26, 12127 (2025).

    Basu, J., Olsson, A., Ferchen, K., et al. ThPOK is a critical multifaceted regulator of myeloid lineage development. Nat. Immunol. 24(8), 1295-1307 (2024).

    Denorme, F., Portier, I., Rustad, J.L., et al. Neutrophil extracellular traps regulate ischemic stroke brain injury. J. Clin. Invest. 132(10), e154225 (2022).

    Clemente-Moragón, A., Martínez-Milla, J., Oliver, E., et al. Metoprolol in critically III patients with COVID-19. J. Am. Coll. Cardiol. 78(10), 1001-1011 (2021).

    Surolia, R., Li, F.J., Wang, Z., et al. NETosis in the pathogenesis of acute lung injury following cutaneous chemical burns. JCI Insight 6(10), e147564 (2021).

    de Andrea, C.E., Ochoa, M.C., Villalba-Esparza, M., et al. Heterogenous presence of neutrophil extracellular traps in human solid tumours is partially dependent on IL-8. J. Pathol. 255(2), 190-201 (2021).

    Lien, T.S., Sun, D.-S., Hung, S.-C., et al. Dengue virus envelope protein domain III induces Nlrp3 inflammasome-dependent NETosis-mediated inflammation in mice. Front. Immunol. 12, 618577 (2021).

    Thålin, C., Aguilera, K., Hall, N.W., et al. Quantification of citrullinated histones: Development of an improved assay to reliably quantify nucleosomal H3Cit in human plasma. J. Thromb. Haemost. 18(10), 2732-2743 (2020).

    Dupuis, J., Sirois, M.G., Rhéaume, E., et al. Colchicine reduces lung injury in experimental acute respiratory distress syndrome. PLoS One 15(12), e0242318 (2020).

    Murthy, P., Singhi, A.D., Ross, M.A., et al. Enhanced neutrophil extracellular trap formation in acute pancreatitis contributes to disease severity and is reduced by chloroquine. Front. Immunol. 10, 28 (2019).