A convenient fluorometric method for screening potential inhibitors of ACE2
Features
  • Screen potential inhibitors for human ACE2
  • Run 43 samples in duplicate
  • Rapid assay; get results in 30 minutes
  • Plate-based fluorescent readout
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ACE2 Inhibitor Screening Assay Kit

Item No. 502100

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • ACEH
  • ACE-related Carboxypeptidase
  • Angiotensin-converting Enzyme 2
  • Angiotensin-converting Enzyme Homolog
  • Metalloprotease MPROT15
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $504

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    Cayman Chemical
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    Product Description

    Cayman’s ACE2 Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for testing potential inhibitors of human ACE2. The assay uses an ACE2-specific fluorogenic substrate, Mca-APK (Dnp). ACE2 cleaves this substrate generating free Mca, which can be easily quantified using a fluorescence plate reader at excitation and emission wavelengths of 320 and 405 nm, respectively. The potent and reversible ACE2 inhibitor MLN-4760 is included as a positive control. Human ACE2 plays an important role in regulating blood pressure. It is also known to bind SARS-CoV-2 spike. This assay can be used as a counter-screen for potential drug candidates to ensure they do not interfere with the normal physiological functions of ACE2.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Gao, B., Zhu, H., Liu, Z., et al. Chemical compositions of Scutellaria baicalensis Georgi. (Huangqin) extracts and their effects on ACE2 binding of SARS-CoV-2 spike protein, ACE2 activity, and free radicals. Int. J. Mol. Sci. 25(4), 2045 (2024).

    Gao, B., Zhu, H., Liu, Z., et al. Chemical compositions of lianqiao (Forsythia suspensa) extracts and their potential health benefits. Pharmaceuticals (Basel) 17(6), 740 (2024).

    Choe, U., Li, Y., Liu, Z., et al. Chemical compositions of dill (Anethum graveolens L.) water and ethanol extracts and their potential in blocking the SARS-CoV‑2 entry into cells and free radical scavenging capacities. ACS Food Sci. Technol. 3(10), 1654-1662 (2023).

    Li, Y., Liu, Z., Zeng, M., et al. Chemical compositions of clove (Syzygium aromaticum (L.) Merr. & L.) extracts and their potentials in suppressing SARS-CoV-2 spike protein-ACE2 binding, inhibiting ACE2, and scavenging free radicals. J. Agric. Food Chem. 70(45), 14403-14413 (2022).

    Yao, Y., Whent, M., Li, Y., et al. Chemical composition of thyme (Thymus vulgaris) extracts, potential inhibition of SARS-CoV-2 spike protein-ACE2 binding and ACE2 activity, and radical scavenging capacity. J. Agric. Food. Chem. 71(49), 19523-19530 (2023).

    Yao, Y., Choe, U., Li, Y., et al. Chemical composition of rosemary (Rosmarinus officinalis L.) extract and its inhibitory effects on SARS-CoV-2 spike protein-ACE2 interaction and ACE2 activity and free radical scavenging capacities. J. Agric. Food Chem. 71(48), 18735-18745 (2023).