For the quantification of PG in culture media
Features
  • Measure total PG levels in tissue culture medium
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure total PG levels down to 30 pg/ml
  • Incubation: 18 hours | Development: 60-90 minutes | Read: Colorimetric at 405-420 nm
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Prostaglandin Screening ELISA Kit

Item No. 514012

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • PG Screening
  • PG Screening EIA
Assay Range
15.6-2,000 pg/ml
Sensitivity
80% B/B0: 30 pg/ml
Cross Reactivity
(Prostaglandin E1) 100%(Prostaglandin E2) 100%(Prostaglandin F1α) 100%(Prostaglandin F2α) 100%(Prostaglandin F3α) 51.3%(Prostaglandin E2 Ethanolamide) 44.0%(6-keto Prostaglandin F1α) 43.6%(8-iso Prostaglandin F2α) 38.4%(8-iso Prostaglandin E2) 28.5%(Prostaglandin D2) 26.6%(8-iso-2,3-dinor Prostaglandin F1α) 20.0%(Prostaglandin E3) 9.5%(Thromboxane B2) 5.0%(12(S)-HHTrE) 0.25%(Leukotriene D4) 0.2%(Arachidonic Acid) <0.01%(Leukotriene B4) <0.01%(Leukotriene C4) <0.01%(Leukotriene E4) <0.01%(Misoprostol) <0.01%(Misoprostol (free acid)) <0.01%(Prostaglandin A1) <0.01%(Prostaglandin A2) <0.01%(Prostaglandin A3) <0.01%(Prostaglandin B1) <0.01%(15-keto Prostaglandin E2) <0.01%(13,14-dihydro-15-keto Prostaglandin F2α) <0.01%
Origin
Animal/Bovine, Animal/Eel, Animal/Mouse, Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 solid wells

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    Product Description

    This assay was developed for screening applications in which the relative amount of prostaglandin (PG) production for a large number of cell culture samples must be determined. Our PGE2 assay (Item No. 514010) is unparalleled in sensitivity and specificity, but the steep standard curve can make screening many samples tedious. The specificity of the PGE2 assay also eliminates quantification of other PGs which may be produced by the cells. The antiserum used in this assay exhibits high cross reactivity for most PGs which will allow quantification of all the PGs in a given sample with a single assay. The broad standard curve minimizes the need for sample dilution. Because this assay will also recognize thromboxane B2 (TXB2), cell culture media must not contain fetal calf serum (FCS). The high concentration of TXB2 in serum (approximately 400 ng/ml) will completely displace the tracer, making PG measurement impossible.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.