For the quantification of testosterone in serum, plasma, tissue culture supernatants and other sample matrices.
Features
  • Measure testosterone in multiple sample types
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure testosterone levels down to 6 pg/ml
  • Incubation: 2 hours | Development: 60-90 minutes | Read: Colorimetric at 405-420 nm
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Testosterone ELISA Kit

Item No. 582701

Exempt Product (Canada)
Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Testosterone EIA Kit
Limit of Detection
3 pg/ml
Assay Range
3.9-500 pg/ml
Sensitivity
6 pg/ml
Cross Reactivity
(140%) 19-Nortestosterone(100%) Testosterone(27.4%) 5α-dihydrotestosterone(18.9%) 5β-dihydrotestosterone(4.7%) Methyl Testosterone(3.7%) Androstenedione(2.2%) 11-keto Testosterone(0.51%) 5-Androstenediol(0.2%) epi-Testosterone(0.14%) Progesterone(0.11%) Testosterone Enanthate(0.05%) Androsterone(0.04%) Androsterone Sulfate(0.03%) Testosterone Sulfate(0.02%) DHEA Sulfate(<0.01%) Estradiol(<0.01%) Testosterone Glucuronide
Origin
Animal/Bovine, Animal/Eel, Animal/Mouse, Animal/Rabbit
Regulatory Information
Canada Exempt Notification
This product is registered as a Canada Test Kit - no additional Health Canada import paperwork or permits required.
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

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    Bioanalysis & Assay Development Services
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    • Bioanalysis and compound screening using Cayman or qualified commercial assay kits
    • Development and qualification of fit-for-purpose assays in a variety of formats and platforms
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    Product Description

    Cayman’s Testosterone ELISA Kit is a competitive assay that can be used for quantification of testosterone in plasma, serum, saliva, tissue culture supernatants, animal feces, and other sample matrices. The assay does not recognize testosterone-17-glucuronide, the metabolite most prevalent in urine, and therefore is not recommended for use with urine samples. The assay has a range from 3.9-500 pg/ml, a sensitivity (80% B/B0) of approximately 6 pg/ml, and a lower limit of detection (LLOD) of 3 pg/ml.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Godiwala, P., Uliasz, T.F., Lowther, K.M., et al. Puberty suppression followed by testosterone therapy does not impair reproductive potential in female mice. Endocrinology 164(11), bqad145 (2023).

    Taves, M.D., Donahue, K.M., Bian, J., et al. Aire drives steroid hormone biosynthesis by medullary thymic epithelial cells. Sci. Immunol. 8(86), eabo7975 (2023).

    Estienne, A., Fréville, M., Bourdon, G., et al. Chronic dietary exposure to a glyphosate-based herbicide results in reversible increase early embryo mortality in chicken. Ecotoxicol. Environ. Saf. 241, 113741 (2022).

    Kuzmanov, A., and Zwiesler-Vollick, J. A discovery-based undergraduate laboratory exercise to investigate the effect of potential endocrine disruptors on sex hormones using human cell culture. Biochem. Mol. Biol. Educ. 50(5), 527-536 (2022).

    Liu, X., Lu, X., Hong, J., et al. Effects of long-term exposure to TDCPP in zebrafish (Danio rerio) - alternations of hormone balance and gene transcriptions along hypothalamus-pituitary axes. Animal Model Exp. Med. 5(3), 239-247 (2022).

    Koike, T., Yoshikawa, M., Ando, H.K., et al. Discovery of soticlestat, a potent and selective inhibitor for cholesterol 24-hydroxylase (CH24H). J. Med. Chem. 64(16), 12228-12244 (2021).

    Bock, S.L., Chow, M.I., Forsgren, K.L., et al. Widespread alterations to hypothalamic-pituitary-gonadal HPG) axis signaling underlie high temperature reproductive inhibition in the eurythermal sheepshead minnow (cyprinodon variegatus). Mol. Cell. Endocrinol. 525:111198, (2021).

    Li, N., Xu, N., Lin, Y., et al. Roles of testosterone and estradiol in mediation of acute neuroendocrine and electroencephalographic effects of sevoflurane during the sensitive period in rat. Front. Endocrinol. (Lausanne) 11, 545973 (2020).

    Shin, Y.Y., Jeong, J.S., Park, M.-N., et al. Regulation of steroid hormones in the placenta and serum of women with preeclampsia. Mol. Med. Rep. 17(2), 2681-2688 (2018).

    Zhu, Y., Hua, R., Zhou, Y., et al. Chronic exposure to mono-(2-ethylhexyl)-phthalate causes endocrine disruption and reproductive dysfunction in zebrafish. Environ. Toxicol. Chem. 35(8), 2117-2124 (2016).

    Zhao, Y., Xie, L., and Yan, Y. Microcystin-LR impairs zebrafish reproduction by affecting oogenesis and endocrine system. Chemosphere 120, 115-122 (2015).

    Liu, X., Ji, K., Jo, A., et al. Effects of TDCPP or TPP on gene transcriptions and hormones of HPG axis, and their consequences on reproduction in adult zebrafish (Danio rerio). Aquat. Toxicol. 134-135, 104-111 (2013).

    Ji, K., Liu, X., Lee, S., et al. Effects of non-steroidal anti-inflammatory drugs on hormones and genes of the hypothalamic-pituitary-gonad axis, and reproduction of zebrafish. J. Hazard. Mater. 254-255, 242-251 (2013).

    Liu, X., Ji, K., and Choi, K. Endocrine disruption potentials of organophosphate flame retardants and related mechanisms in H295R and MVLN cell lines and in zebrafish. Aquat. Toxicol. 114-115, 173-181 (2012).

    Liu, C., Deng, J., Yu, L., et al. Endocrine disruption and reproductive impairment in zebrafish by exposure to 8:2 fluorotelomer alcohol. Aquat. Toxicol. 96(1), 70-76 (2010).

    Liu, C., Yu, L., Deng, J., et al. Waterborne exposure to fluorotelomer alcohol 6:2 FTOH alters plasma sex hormone and gene transcription in the hypothalamic-pituitary-gonadal (HPG) axis of zebrafish. Aquat. Toxicol. 93(2-3), 131-137 (2009).