A cell-based assay for studying modulators of cellular glucose uptake
Features
  • Used for the direct detection of glucose taken up in cells using the fluorescently labeled deoxyglucose analog 2-NBDG
  • Flexible format can be analyzed with either flow cytometry or fluorescence microscopy
  • Utilizes buffers compatible with Western blot, allowing subsequent testing of intact cells
  • Apigenin (Item No. 10010275) a flavinoid and a known inhibitor of glucose transport is included as a control
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Glucose Uptake Cell-Based Assay Kit

Item No. 600470

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    1 ea

    $385

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    Cayman Chemical
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    Product Description

    Cayman’s Glucose Uptake Cell-Based Assay Kit provides a convenient tool for studying modulators of cellular glucose uptake. The kit employs NBD-glucose (2-NBDG), a fluorescently labeled deoxyglucose analog, as a probe for the detection of glucose taken up by cultured cells. Researchers interested in cellular metabolism, such as cancer biologists, immunologists, physiologists, and others, will find this kit a robust tool for measuring energy consumption in living cells.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Sonntag, K.C., Ryu, W.I., Amirault, K.M., et al. Late-onset Alzheimer’s disease is associated with inherent changes in bioenergetics profiles. Sci. Rep. 7(1), 14038 (2017).

    Seo, W.-D., Lee, J.H., Jia, Y., et al. Saponarin activates AMPK in a calcium-dependent manner and suppresses gluconeogenesis and increases glucose uptake via phosphorylation of CRTC2 and HDAC5. Bioorg. Med. Chem. Lett. 25(22), 5237-5242 (2015).

    Shajahan-Haq, A.N., Cook, K.L., Schwartz-Roberts, J.L., et al. MYC regulates the unfolded protein response and glucose and glutamine uptake in endocrine resistant breast cancer. Mol. Cancer 13, 239 (2014).