A novel method to assess CYP2C9 induction
Features
  • A 96-well plate coated with DNA encoding a CYP2C9 promoter-regulated reporter enzyme (SEAP) and two nuclear receptors, GRα and HNF4α
  • Cells grown on the complex will express the two nuclear receptors and the CYP2C9 promoter-regulated SEAP
  • GR-mediated CYP2C9 inducers will activate SEAP expression
  • SEAP secreted into the supernatant cleaves a luminescence-based substrate
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CYP2C9 Induction Reporter Assay Kit

Item No. 601120

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 tests

    $511

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    Product Description

    Early screening of drug candidate’s potential to induce or inhibit CYP2C9 is a critical step in the drug development process. The CYP2C9 Induction Reporter Assay Kit is a novel method to evaluate CYP2C9 induction by drug candidates. Cayman's CYP2C9 Induction Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing a Secreted Alkaline Phosphatase (SEAP) reporter regulated by the human CYP2C9 gene promoter. Cells grown on the CYP2C9 Reporter Reverse Transfection Strip Plate will introduce the reporter gene and express GRα as well as HNF4α. Test compounds that promote binding of the nuclear receptors to the CYP2C9 promoter initiates SEAP expression. SEAP activity is subsequently measured using a luminescence readout. The kit is simple to use and can be easily adapted to high-throughput screening for potential nuclear receptor-mediated CYP2C9 inducers.

    WARNING This product is not for human or veterinary use.