An image-based assay for the visualization and quantification of NETosis kinetics
Features
  • Visualize and quantify NET formation kinetically ex vivo and in vitro
  • Cell-impermeable DNA dye detects extruded DNA
  • Includes reagents for enrichment of neutrophils from human blood, staining of cells, and induction of NETosis
  • Cell staining and NETosis induction is adaptable to multiple species
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NETosis Imaging Assay Kit

Item No. 601750

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $545

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    NET Formation Screening & Analysis Services
    Our experts are here to help.
    • Detection of NET formation ex vivo and screening for modulators using:
      • High-content imaging
      • Enzymatic detection
      • Citrullination/carbamylation detection
    • Experienced scientists skilled in neutrophil biology, isolation, and handling
    LEARN MORE
    Product Description

    Cayman’s NETosis Imaging Kit provides a simple staining protocol for visualizing the process of NETosis kinetically ex vivo and in vitro. A cell-permeable DNA dye allows visualization of the dynamic changes of the nucleus during the process of NETosis, while a cell-impermeable dye detects the extruded DNA. Combining the fluorescence visualization of these dyes with brightfield imaging in a high-content platform allows for exquisite differentiation between different modes of cell death. The amount of each reagent provided is sufficient for two 96-well plates.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Fisher, K.L., Mpotje, T., Nargan, K., et al. NETosis associated with human TB lung tissue destruction and diease pathogenesis. EMBO Mol. Med. (2026).

    Harman, R.M., Sipka, A., Oxford, K.A., et al. The mammosphere-derived epithelial cell secretome modulates neutrophil functions in the bovine model. Front. Immunol. 15, 1367432 (2024).