Colorimetric assay for the continuous monitoring of SAM-dependent methyltransferases
Features
  • Continuous assay to monitor SAM-dependent methyltransferases
  • Assay 45 samples in duplicate
  • Plate-based colorimetric measurement (515 nm)
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Methyltransferase Colorimetric Assay Kit

Item No. 700140

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • MT
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $715

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    Product Description

    Cayman’s Methyltransferase Colorimetric Assay Kit is a continuous enzyme-coupled assay that can monitor SAM-dependent methyltransferases.1 The assay scheme outlines the general scheme of the assay. The removal of the methyl group from SAM generates S-adenosylhomocysteine (AdoHcy), which is rapidly converted to S-ribosylhomocysteine and adenine by AdoHcy nucleosidase. This rapid conversion prevents the buildup of AdoHcy and its feedback inhibition on the methylation reaction. Finally, the adenine is converted to hypoxanthine, by adenine deaminase, which in turn is converted to urate and hydrogen peroxide (H2O2). The rate of production of H2O2 is measured with the colorimetric reagents, 3,5-dichloro-2-hydroxybenzenesulfonic acid and 4-aminoantipyrine, by an increase in absorbance at 515 nm. The assay is supplied with AdoHcy as a positive control. The assay can be used with any purified SAM-dependent methyltransferase.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Dorgan, K.M., Wooderchak, W.L., Wynn, D.P., et al. An enzyme-coupled continuous spectrophotometric assay for S-adenosylmethionine-dependent methyltransferases. Anal. Biochem. 350, 249-255 (2006).