A convenient method of detecting ALT activity in serum, plasma, tissue samples, and cell lysates
Features
  • Measure ALT activity in serum, plasma, tissue samples, and cell lysates
  • Assay 47 samples in duplicate
  • Measure ALT activity down to 0.006 U/ml
  • Plate-based colorimetric measurement (340 nm)
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Alanine Transaminase Colorimetric Activity Assay Kit

Item No. 700260

Technical Information
Synonyms
  • ALT
  • Alanine Aminotransferase
  • ALAT
  • Serum Pyruvic Transaminase
  • sGPT
Limit of Detection
0.006 U/ml or 0.1 SI U/ml
Origin
Animal/Pig
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    Product Description

    Cayman’s Alanine Transaminase (ALT) Colorimetric Activity Assay Kit provides a convenient method of detecting ALT activity in serum, plasma, tissue samples, and cell lysates. Measurement of the ALT activity is carried out by monitoring the rate of NADH oxidation in a coupled reaction system employing lactate dehydrogenase (LDH). The oxidation of NADH to NAD+ is accompanied by a decrease in absorbance at 340 nm. Under circumstances in which the ALT activity is rate limiting, the rate decrease is directly proportional to the ALT activity in the sample.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

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