A colorimetric and fluorometric assay for the quantification of uric acid in plasma, serum, and urine
Features
  • Measure uric acid in plasma, serum, and urine
  • Assay 40 samples in duplicate or 26 samples in triplicate
  • Assay Range: 5-100 μM (fluorometric) or 5-1,000 μl (colorimetric)
  • Plate-based fluorometric measurement (ex 530-540 nm, em 585-595 nm) or colorimetric measurement (570 nm)
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Uric Acid Assay Kit

Item No. 700320

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Urate Assay Kit
Emission
585-595 nm (fluorometric)
Excitation
530-540 nm (fluorometric)
Limit of Detection
0.18 µM (fluorometric); 0.45 µM (colorimetric)
Assay Range
5-100 μM (fluorometric); 5-1,000 μM (colorimetric)
Lower Limit of Quantification
5 µM (fluorometric); 50 µM (colorimetric)
Origin
Plant/Armoracia rusticana
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    96 wells

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    Cayman Chemical
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    Product Description

    Cayman’s Uric Acid Assay Kit provides fluorescence- or colorimetric-based methods for detecting uric acid in plasma, serum, and urine. In the assay, uricase catalyzes the conversion of uric acid to allantoin, hydrogen peroxide (H2O2), and carbon dioxide. H2O2, in the presence of horseradish peroxidase, reacts stoichiometrically with ADHP (10-acetyl-3,7-dihydroxyphenoxazine) to produce the highly fluorescent compound resorufin. Resorufin fluorescence can be analyzed with an excitation wavelength between 530-540 nm and an emission wavelength between 585-595 nm. Alternatively, the absorbance of resorufin can be measured at 570 nm.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.