L-Lactate Assay Kit | Cayman Chemical

L-Lactate Assay Kit

Item № 700510
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SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 wells $325.00 0.00

Pricing updated 2018-05-25. Prices are subject to change without notice.

Description
Features
  • Measure L-lactate in biological samples such as serum, plasma, whole blood, urine, saliva, and cell culture
  • Assay 40 samples in duplicate
  • Assay Range: 25 μM-1 mM
  • Plate-based fluorometric measurement (ex 530-540 nm, em 585-595 nm)

L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism. The lactate to pyruvate ratio reflects the redox state of the cell and describes the balance between NAD+ and NADH, which is dependent on the interconversion of lactate and pyruvate via lactate dehydrogenase (LDH). Monitoring lactate levels is, therefore, a good way to evaluate the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Cayman’s L-Lactate Assay provides a fluorescence-based method for detecting L-lactate in biological samples such as serum, plasma, blood, urine, and saliva. It can also be utilized to determine intracellular and extracellular lactate concentrations in cell culture samples. In the assay, lactate dehydrogenase catalyzes the oxidation of lactate to pyruvate, along with the concomitant reduction of NAD+ to NADH. NADH reacts with the fluorescent substrate to yield a highly fluorescent product. The fluorescent product is analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Citations

Angelin, A., Gil-de-Gómez, L., Dahiya, S., et al. Foxp3 reprograms T cell metabolism to function in low-glucose, high-lactate environments. Cell. Metab. 25(6), 1282-1293 (2017).

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You may be eligible to receive a free sample of L-Lactate Assay Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640