A fluorescence-based method for detecting lipase activity in plasma, serum, tissue homogenates, and cell culture samples
Features
  • Measure lipase activity in plasma, serum, tissue homogenates, and cell lysates
  • Assay 20 samples in duplicate
  • Measure lipase activity down to 0.1 U/ml
  • Plate-based fluorometric measurement (ex 380-390 nm, em 510-520 nm)
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Lipase Activity Assay Kit

Item No. 700640

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Limit of Detection
0.1 U/ml (±0.05 U/ml) lipase
Origin
Animal/Bovine
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    96 wells

    $359

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    Cayman Chemical
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    Product Description

    Lipases perform essential roles in the digestion, transportation, and processing of dietary lipids by controlling the clearance of triglyceride-rich lipoproteins from the circulation. Manipulating lipolysis has therapeutic potential in the metabolic disorders frequently associated with obesity. Cayman’s Lipase Activity Assay provides a fluorescence-based method for detecting lipase activity in plasma, serum, tissue homogenates, and cell culture samples. In the assay, lipase hydrolyzes arachidonoyl-1-thioglycerol to arachidonic acid and thioglycerol. Thioglycerol reacts with the thiol fluorometric detector to yield a highly fluorescent product which can be analyzed with an excitation wavelength of 380-390 nm and an emission wavelength of 510-520 nm.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Aggarwal, G., Zarrow, J.E., Mashhadi, Z., et al. Symmetrically substituted dichlorophenes inhibit N-acyl-phosphatidylethanolamine phospholipase D. The Journal of Biological Chemisty 295(21), 7289-7300 (2020).