A sensitive fluorometric assay for assaying β-HB from plasma, serum, urine, tissue homogenates, and cell lysate samples
Features
  • Measure β-HB from plasma, serum, urine, cell lysates, and tissue homogenates
  • Assay 20 samples in duplicate
  • Assay Range: 2.5-50 μM
  • Plate-based fluorometric measurement (ex 530-540 nm, em 585-595 nm)
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β-Hydroxybutyrate (Ketone Body) Fluorometric Assay Kit

Item No. 700740

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • β-HB
  • 3-Hydroxybutyric Acid
Limit of Detection
1.2 µM
Lower Limit of Quantification
1.5 µM
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    96 wells

    $377

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    Cayman Chemical
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    Product Description

    Cayman’s β-HB (Ketone Body) Fluorometric Assay Kit provides a simple, reproducible, and sensitive tool for assaying β-HB from plasma, serum, urine, tissue homogenates, and cell lysates. The method for β-HB determination is based upon the oxidation of D-3-Hydroxybutyrate to acetoacetate by the enzyme 3-hydroxybutyrate dehydrogenase.16 Concomitant with this oxidation, the cofactor NAD+ is reduced to NADH. NADH reacts with the fluorometric developer to yield a highly fluorescent product which can be analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm. The fluorescence is directly proportional to the β-HB concentration.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.