A colorimetric assay for the detection of aspartate aminotransferase activity in serum, plasma, tissue samples and cell lysates
Features
  • Measure AST activity in serum, plasma, tissue samples, and cell lysates
  • Assay 31 samples in triplicate and 46 in duplicate
  • Measure AST activity down to 0.011 U/ml
  • Plate-based colorimetric measurement (340 nm)
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Aspartate Aminotransferase Colorimetric Activity Assay Kit

Item No. 701640

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Aspartate Aminotransaminase
  • AST
  • Serum Glutamatic Oxaloacetic Transaminase
  • SGOT
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $385

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    Cayman Chemical
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    Product Description

    Cayman’s Aspartate Aminotransferase Colorimetric Activity Assay Kit provides a convenient method of detecting AST activity in serum, plasma, tissue samples, and cell lysates. Measurement of the AST activity is carried out by monitoring the rate of NADH oxidation in a coupled reaction system employing malate dehydrogenase (MDH). The oxidation of NADH to NAD+ is accompanied by a decrease in absorbance at 340 nm. Under circumstances in which the AST activity rate is limiting, the rate of decrease is directly proportional to the AST activity in the sample. Lactate dehydrogenase (LDH) is added to prevent interference from endogenous pyruvate normally present in serum.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

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    Padilla, M.S., Mrksich, K., Wang, Y., et al. Branched endosomal disruptor (BEND) lipids mediate delivery of mRNA and CRISPR-Cas9 ribonucleoprotein complex for hepatic gene editing and T cell engineering. Nat. Commun. 16(1), 996 (2025).

    Han, X., Xu, J., Xu, Y., et al. In situ combinatorial synthesis of degradable branched lipidoids for systemic delivery of mRNA therapeutics and gene editors. Nat. Commun. 15(1), 1762 (2024).

    Han, X., Alameh, M.G., Gong, N., et al. Fast and facile synthesis of amidine-incorporated degradable lipids for versatile mRNA delivery in vivo. Nat. Chem. 16(10), 1687-1697 (2024).

    Yu, M., Dorsey, K.H., Halseth, T., et al. Enhancement of anti-inflammatory effects of synthetic high-density lipoproteins by incorporation of anionic lipids. Mol. Pharm. 20(11), 5454-5462 (2023).

    Han, X., Gong, N., Xue, L., et al. Ligand-tethered lipid nanoparticles for targeted RNA delivery to treat liver fibrosis. Nat. Commun. 14(1), 75 (2023).

    Madhavan, A., Kok, B.P., Rius, B., et al. Pharmacologic IRE1/XBP1s activation promotes systemic adaptive remodeling in obesity. Nat. Commun. 13(1), 608 (2022).

    Riley, R.S., Kashyap, M.V., Billingsley, M.M., et al. Ionizable lipid nanoparticles for in utero mRNA delivery. Sci. Adv. 7(3), eaba1028 (2021).

    Wei, W., Lyu, X., Markhard, A.L., et al. PTER is a N-acetyltaurine hydrolase that regulates feeding and obesity. Nature 633(8028), 182-188 (2023).