A colorimetric method for rapid detection of total and direct bilirubin levels in serum.
Features
  • Measure both total and direct bilirubin in serum
  • Assay 52 samples in duplicate
  • Detect bilirubin levels down to 0.045 mg/dl
  • Plate-based colorimetric measurement (600 nm and 525 nm)
  • Rapid test, obtain results in 30 minutes
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Total and Direct Bilirubin Colorimetric Assay Kit

Item No. 701720

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Limit of Detection
0.036 µg/well or 0.071 mg/dl (Total Bilirubin)
Assay Range
0.045-8 mg/well (0.022-4 μg/well) (Total and Direct Bilirubin)
Lower Limit of Quantification
0.125 µg/well or 0.25 mg/dl (Total Bilirubin)
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea

    $532

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    Cayman Chemical
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    Product Description

    Cayman’s Total and Direct Bilirubin Colorimetric Assay provides a rapid, reliable, and reproducible colorimetric method for measuring total and direct bilirubin levels in serum. This kit uses a modified Jendrassik-Gróf method, in which total bilirubin concentration is determined by diazo salt in the presence of a catalyst and direct bilirubin is determined in the absence of the catalyst.1,2 The Jendrassik-Gróf method is a widely used clinical method.3 However, the results may not be comparable to the results of analytical methods such as mass spectrometry or NMR, and this kit is designed for research use only (not to be used for clinical purposes).

    The assay has a dynamic range of 0.045-8 mg/dl (0.022-4 μg/well) for both total and direct bilirubin.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lo, D.H., and Wu, T.W. Assessment of the fundamental accuracy of the Jendrassi-Gróf total and direct bilirubin assays. Clin. Chem. 29(1), 31-36 (1983).

    2. Garber, C.C. Jendrassik Gróf analysis for total and direct bilirubin inserum with a centrifugal analyzer. Clin. Chem. 27(8), 1410-1416 (1981).

    3. Tietz, N.W. Clinical Guide to Laboratory Tests. 268-273 (1995).

    Product Citations

    Han, X., Gong, N., Xue, L., et al. Ligand-tethered lipid nanoparticles for targeted RNA delivery to treat liver fibrosis. Nat. Commun. 14(1), 75 (2023).