A convenient fluorometric method of screening human ENPP1 inhibitors
Features
  • Screen for inhibitors of human ENPP1
  • Includes recombinant human ENPP1 and positive control ENPP1 inhibitor C Assay Reagent
  • Assay 45 samples in duplicate or 29 samples in triplicate
  • Plate-based fluorometric measurement (Ex/Em = 485/520 nm)
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ENPP1 Fluorescent Inhibitor Screening Assay Kit

Item No. 702090

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Ectonucleotide Pyrophosphatase/Phosphodiesterase I
  • Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 1
Origin
Animal/Bovine
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    Product Description

    Cayman’s ENPP1 Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of human ENPP1, an ectonucleotide pyrophosphatase and phosphodiesterase implicated in multiple biological pathways, including the cGAS/STING pathway, and bone mineralization. The assay uses an ENPP1-specific fluorogenic substrate, Tokyo Green™-mAMP (TG-mAMP). ENPP1 cleaves this substrate generating free Tokyo Green™, which can be easily quantified using a fluorescence plate reader at excitation and emission wavelengths of 485 and 520 nm, respectively. The potent and reversible ENPP1 inhibitor ENPP1 Inhibitor C Assay Reagent is included as a positive control.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.