A colorimetric assay for the quantification of NADP+, NADPH, and their ratio in cell lysates.
Features
  • Simple and convenient assay for quantifying pyridine nucleotides
  • Room temperature, endpoint colorimetric 96-well plate method
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NADPH/NADP Ratio Assay Kit

Item No. 702450

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Limit of Detection
0.02 µM
Assay Range
0-1.5 µM
Origin
Animal/Bovine, Microorgansim
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    96 wells

    $495

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    Cayman Chemical
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    Product Description

    Cayman’s NADPH/NADP Ratio Assay Kit provides a colorimetric method for measuring NADP+, NADPH, and their ratio in cell culture extracts. In this assay, NADP+ is enzymatically converted to NADPH by glucose-6-phosphate dehydrogenase (G6PDH), which leads to the reduction of the tetrazolium salt substrate WST-8 to a highly colored formazan that absorbs at 450 nm. The amount of formazan produced is proportional to the amount of NADP+ and NADPH in the matrix. To measure NADPH specifically, samples are heated to degrade NADP+. The NADP+ concentration is then calculated by subtracting the signal obtained from the heated sample from that of the corresponding non-heated sample. This assay has a range of 0-1.5 µM and a lower limit of detection (LLOD) of 0.02 µM.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.