For the measurement of hydroperoxides
Features
  • Measure LPOs in tissues, cultured cells, plant materials, foods, and biological fluids
  • Assay 40 samples in duplicate
  • Assay Range: 0.25-5 nmol
  • Plate-based colorimetric measurement (500 nm)
  • Requires reusable glass plate
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Lipid Hydroperoxide (LPO) Assay Kit

Item No. 705002

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Shipping & Storage Information
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 dtn

    $358

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    Product Description

    Quantification of lipid peroxidation is essential to assess the role of oxidative injury in pathophysiological disorders.1,2,3 Lipid peroxidation results in the formation of highly reactive and unstable hydroperoxides of both saturated and unsaturated lipids. Our Lipid Hydroperoxide Assay Kit measures the hydroperoxides directly utilizing the redox reactions with ferrous ions.4 An easy to use quantitative extraction method was developed to extract lipid hydroperoxides into chloroform, and the extract is directly used in the assay. This procedure eliminates any interference caused by hydrogen peroxide or endogenous ferric ions in the sample and provides a sensitive and reliable assay for lipid peroxidation. This kit is designed for use with either a single-tube spectrophotometer to read the results or with a 96 well microplate reader. The plate used with the microplate reader is a reusable glass plate which is available with the purchase of Item No. 705003. The range of the assay is 0.25-5 nmol hydroperoxide per tube.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Cross, C.E., Halliwell, B., Borish, E.T., et al. Oxygen radicals and human disease. Ann. Intern. Med. 107, 526-545 (1987).

    2. Halliwell, B. Oxidative stress, nutrition and health. Experimental strategies for optimization of nutritional antioxidant intake in humans. Free Radic. Res. 25(1), 57-74 (1996).

    3. Porter, N.A., Mills, K.A., and Caldwell, S.E. Mechanisms of free radical oxidation of unsaturated lipids. Lipids 30(4), 277-290 (1995).

    4. Roomi, M.W., and Hopkins, C.Y. Some reactions of sterculic and malvalic acids. A new source of malvalic acid. Can. J. Biochem. 48, 759-762 (1970).

    Product Citations

    Mazereeuw, G., Herrmann, N., Andreazza, A.C., et al. Baseline oxidative stress is associated with memory changes in omega-3 fatty acid treated coronary artery disease patients. Cardiovasc. Psychiatry Neurol. 3674371, (2017).

    Alarifi, S., Ali, H., Alkahtani, S., et al. Regulation of apoptosis through bcl-2/bax proteins expression and DNA damage by nano-sized gadolinium oxide. Int. J. Nanomedicine 12, 4541-4551 (2017).

    Karthivashan, G., Arulselvan, P., Alimon, A.R., et al. Competing role of bioactive constituents in Moringa oleifera extract and conventional nutrition feed on the performance of Cobb 500 broilers. Biomed Res. Int. 970398, (2015).

    Faisal, M., Saquib, Q., Alatar, A.A., et al. Phytotoxic hazards of NiO-nanoparticles in tomato: A study on mechanism of cell death. J. Hazard. Mater. 250-251, 318-332 (2013).

    Liu, J.-Q., Lee, T.-F., Bigam, D.L., et al. Effects of post-resuscitation treatment with N-acetylcysteine on cardiac recovery in hypoxic newborn piglets. PLoS One 5(12), e15322 (2010).

    Liu, J.Q., Lee, T.F., Bigam, D.L., et al. Effects of post-resuscitation treatment with N-acetylcysteine on cardiac recovery in hypoxic newborn piglets. PLoS One 5(12), e15322 (2010).