A quantitative assay for the direct measurement of lipid hydroperoxides
Features
  • Measure LPOs in tissues, cultured cells, plant materials, foods, and biological fluids
  • Assay 40 samples in duplicate
  • Assay Range: 0.25-5 nmol
  • Plate-based colorimetric measurement (500 nm)
  • Includes reusable glass plate
Related Products
Alternative(s)
705002Lipid Hydroperoxide (LPO) Assay Kit
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Lipid Hydroperoxide (LPO) Assay Kit (96 well)

Item No. 705003

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Shipping & Storage Information
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $505

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    Product Description

    Quantification of lipid peroxidation is essential to assess the role of oxidative injury in pathophysiological disorders.1,2,3 Lipid peroxidation results in the formation of highly reactive and unstable hydroperoxides of both saturated and unsaturated lipids. Our Lipid Hydroperoxide Assay Kit measures the hydroperoxides directly utilizing the redox reactions with ferrous ions.4 An easy to use quantitative extraction method was developed to extract lipid hydroperoxides into chloroform, and the extract is directly used in the assay. This procedure eliminates any interference caused by hydrogen peroxide or endogenous ferric ions in the sample and provides a sensitive and reliable assay for lipid peroxidation. This kit is available in two formats: 100 dtn, which is designed to be read using a single-tube spectrophotometer, and 96 wells, which is designed to be read using a 96-well microplate reader. The 96 well kit contains a special glass plate which is resistant to organic solvents used in the assay; this is the only difference between the two kit types.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Cross, C.E., Halliwell, B., Borish, E.T., et al. Oxygen radicals and human disease. Ann. Intern. Med. 107, 526-545 (1987).

    2. Halliwell, B. Oxidative stress, nutrition and health. Experimental strategies for optimization of nutritional antioxidant intake in humans. Free Radic. Res. 25(1), 57-74 (1996).

    3. Porter, N.A., Mills, K.A., and Caldwell, S.E. Mechanisms of free radical oxidation of unsaturated lipids. Lipids 30(4), 277-290 (1995).

    4. Roomi, M.W., and Hopkins, C.Y. Some reactions of sterculic and malvalic acids. A new source of malvalic acid. Can. J. Biochem. 48, 759-762 (1970).

    Product Citations

    Matsumura, T., Uryu, O., Matsuhisa, F., et al. N-acetyl-L-tyrosine is an intrinsic triggering factor of mitohormesis in stressed animals. EMBO Rep. 21(5), e49211 (2020).

    Esgalhado, M., Stockler-Pinto, M.B., de França Cardozo , L.F.M., et al. Effect of acute intradialytic strength physical exercise on oxidative stress and inflammatory responses in hemodialysis patients. Kidney Res. Clin. Pract. 34(1), 35-40 (2015).

    Albaayit, S.F., Abba, Y., Rasedee, A., et al. Effect of Clausena excavata Burm. f. (Rutaceae) leaf extract on wound healing and antioxidant activity in rats. Drug Des. Devel. Ther. 9, 3507-3518 (2015).

    Tbahriti, H.F., Kaddous, A., Bouchenak, M., et al. Effect of different stages of chronic kidney disease and renal replacement therapies on oxidant-antioxidant balance in uremic patients. Biochem. Res. Int. 2013, 358985 (2013).

    Foussal, C., Lairez, O., Calise, D., et al. Activation of catalase by apelin prevents oxidative stress-linked cardiac hypertrophy. FEBS Lett. 584(11), 2363-2370 (2010).