A fluorogenic substrate of α-L-iduronidase and iduronate-2-sulfatase
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4-Methylumbelliferyl-α-L-Iduronide 2-sulfate (sodium salt)

Item No. 9001600

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2-(hydrogen sulfate) 4-methyl-2-oxo-2H-1-benzopyran-7-yl α-L-idopyranosiduronic acid, disodium salt
CAS Number
2181774-46-7
Synonyms
  • 4-Methylumbelliferyl-α-L-Idopyranosiduronic Acid 2-sulfate
  • 4-MU-α-IdoA 2-sulfate
Molecular Formula
C16H14O12S • 2Na
Formula Weight
476.3
Purity
≥98%
Emission
445-454 nm
Excitation
330 (pH 4.6), 370 (pH 7.4), 385 (pH 10.4)
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlPBS (pH 7.2): 10 mg/ml
λmax
319 nm
SMILES
O=C1C=C(C)C2=C(C=C(O[C@H]3[C@H](OS([O-])(=O)=O)[C@@H](O)[C@H](O)[C@H](C([O-])=O)O3)C=C2)O1.[Na+].[Na+]
InChi Code
InChI=1S/C16H16O12S.2Na/c1-6-4-10(17)26-9-5-7(2-3-8(6)9)25-16-14(28-29(22,23)24)12(19)11(18)13(27-16)15(20)21;;/h2-5,11-14,16,18-19H,1H3,(H,20,21)(H,22,23,24);;/q;2*+1/p-2/t11-,12-,13+,14+,16+;;/m0../s1
InChi Key
BGWMBPGUPUSUIU-CEWNINSASA-L
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    500 µg

    $236

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    1 mg

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    Product Description

    4-Methylumbelliferyl-α-L-iduronide 2-sulfate (4-MU-α-IdoA 2-sulfate) is a fluorogenic substrate of α-L-iduronidase that has been used in an assay to detect Hurler syndrome.1 It is also used as a substrate for iduronate-2-sulfatase in tests for Hunter disease.2,3 For these latter tests, the initial enzymatic product, 4-MU-α-IdoA (Item No. 19543) can be measured by mass spectrometry, or it can be hydrolyzed with α-L-iduronidase to liberate the fluorophore 4-MU, which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively.4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Matalon, R., and Dorfman, A. Hurler’s syndrome, an -L-iduronidase deficiency. Biochem. Biophys. Res. Commun. 47(4), 959-964 (1972).

    2. Lee, K., Jun, S.-H., Song, S.H., et al. Direct assay of iduronate-2-sulfatase for Hunter disease using UPLC-tandem mass spectrometry and fluorogenic substrate. Clin. Biochem. 48(18), 1350-1353 (2015).

    3. Voznyi, Y.V., Keulemans, J.L., and Van Diggelen, O.P. A fluorimetric enzyme assay for the diagnosis of MPS II (Hunter disease). J. Inherit. Metab. Dis. 24(6), 675-680 (2001).

    4. Zhi, H., Wang, J., Wang, S., et al. Fluorescent properties of hymecromone and fluorimetric analysis of hymecromone in compound dantong capsule. J. Spectrosc. 147128 (2013).