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Antibody Services

With a long-standing history of antibody production and making high-quality ELISA kits using many of the antibodies we produce, Cayman’s scientists are eager to lend their expertise to your antibody-related projects. Our scientists can produce high-quality antigens and antibodies suitable for a variety of applications, including ELISA, flow cytometry (FC)/FACS, immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), multiplex-based assays, TR-FRET, and Western blot (WB).

Our Services

Antigen Synthesis (1-2 months)

Cayman has the capability to provide peptide, protein, or small molecule immunogens.

We offer:

Solid-phase peptide synthesis (5-20 residues, 5 mg at ≥70% purity, mass verified by MS)
Addition of terminal PEG-cysteine for conjugation chemistry
Immunogen conjugation to carrier of choice (KLH, BSA, etc.)
Modified peptides (citrullinated, methylated, phosphorylated, acetylated, carbamylated, click chemistry-modified, and more)
Custom synthesis of small molecule conjugates for use as antigens/immunogens

We can use a wide array of different antigens/immunogens for monoclonal or polyclonal antibody production, including:

Peptides (including carrier-conjugated peptides)
Proteins
Small molecules (including carrier-conjugated small molecules)
Over-expressing cell lines
Toxins
Polysaccharides

Monoclonal Antibody Production (4-7 months from immunization)

Cayman can produce highly specific monoclonal antibodies. We offer two levels of monoclonal antibody production services, as well as hybridoma banking services. At each level of service, customers can provide their own immunogen or have Cayman customize the immunogen of your choice.

	Level 1	Level 2
Cost	$$$	$$$-$$$$
Time frame (from immunization)	4-5 months	5-7 months
Immunization of three mice
Mouse response titered after second bleed
Fusion followed by primary screen
Two rounds of subcloning for up to 10 parental fusions, screening by ELISA (option to screen additional parentals)
Stable and positive clones frozen and sent to customer
Expansion and cell banking of clones (cell banking for five tubes per clone)
Cell growth scaled up and 200 ml of supernatant purified and sent to customer
Milligram-to-gram scale available for growth/purification upon request

Polyclonal Antibody Production (3-4 months)

Cayman offers three levels of polyclonal antibody production services to meet your specific needs for highly sensitive polyclonal antibodies.

	Level 1	Level 2	Level 3
Synthesis of peptide antigen and conjugation to carrier of choice	Customer provides antigen for immunization
Immunization of antigen into two rabbits (option for additional rabbits)
Screening of first bleeds by ELISA three months post-immunization
Purification of 25 ml serum
Cost per antigen	$	$$	$$$

Recombinant Antibody Production

Services offered include:

Cloning and plasmid production
Transfection and high-throughput electroporation (MaxCyte STx)
Stable cell line generation
Cell culture
Purification
Fit-for-purpose application development/validation

Epitope Tag Antibody Production

Cayman can generate antibodies to epitope tags that are not restricted by current patents or licensing.

Antibody Purification

We offer a variety of antibody purification methods:

Protein A & G
Affinity
Precipitation
Customer-defined

Antibody Conjugation (2-4 weeks)

We offer custom conjugation of antibodies with a variety of tags and labels, including HRP, alkaline phosphatase (AP), biotin, FITC, phycoerythrin (PE), and allophycocyanin (APC). We can conjugate a minimum of 100 μg of antibody, with bulk quantities available.

Antibody Characterization

Our services include:

Epitope mapping
Surface plasmon resonance (SPR) to measure binding kinetics using the Biacore™ 8K
Antibody pair screening for assay development

Validation/QC

We offer antibody validation for a variety of applications, including:

ELISA
FC
IF
IHC
IP
WB

Blocking Peptide Production

Peptide antigens produced for customer development/immunization projects can be supplied to serve as experimental blocking reagents.

Affinity Resin Production

Cayman can provide custom development and manufacturing of immunoaffinity resins designed to purify and/or enrich for proteins and small molecules. We have extensive experience developing antibody-based capture sorbents using antibodies against:

Post-translational modifications (PTMs)
Low-abundance targets
Lipids
Small molecules

We can also use antibodies provided by the customer for tailor-made workflows.

Immunoprecipitation

We offer immunoprecipitation (IP) services for analysis of protein interactions. Our services include:

Custom matrices using validated antibodies for target pulldown from cell or tissue samples
LC-MS/MS (Orbitrap), ELISA, or Western blot (WB) analysis of IP samples
In-house cell culture and drug/compound treatment capabilities in our BSL2 facilities

Immunohistochemistry

Immunohistochemistry (IHC) antibody testing against formalin-fixed paraffin-embedded (FFPE) samples with histology sectioning capabilities on a wide range of tissue types.

Pricing

Pricing varies based on the service(s) requested. Request more information, including project-specific pricing, by submitting the “Contact Us” form.

Proteomic Profiling

Mass spectrometry-based quantitative proteomics.

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CRO & CDMO SERVICES