96 Well Assay for measurement of PPARα, δ, γ
Features
  • A sensitive, non-radioactive method of detecting PPARα, PPARγ, and PPARδ/β from whole cell lysates
  • 96-well plate format replaces EMSAs
  • Capture the transcription factor using a specific dsDNA sequence bound to the plate
  • Detect the dsDNA-bound transcription factor with specific antibodies in an ELISA format
  • Single 96-well plate that measures all three PPAR isoforms
  • Nuclear Extraction Kit (Item No. 10009277) available to aid in the isolation of nuclear and cytoplasmic fractions from cell lysates or tissue homogenates
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PPARα, δ, γ Complete Transcription Factor Assay Kit

Item No. 10008878

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Origin
Animal/Bovine, Animal/Goat, Animal/Rabbit
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $543

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    Cayman Chemical
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    Product Description

    Cayman’s PPARα, δ, γ Complete Transcription Factor Assay Kit is a sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A specific double stranded DNA (dsDNA) sequence containing the PPAR response element is immobilized onto the wells of a 96-well plate. PPARs contained in a sample bind specifically to the immobilized PPAR response element and are detected by addition of specific primary antibodies directed against the individual PPAR isoforms. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. Cayman’s PPARα, δ, γ Complete Transcription Factor Assay Kit is designed to investigate all three PPAR isoforms in 12 samples on a single plate.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Thering, B.J., Bionaz, M., and Loor, J.J. Long-chain fatty acid effects on peroxisome proliferator-activated receptor-a-regulated genes in Madin-Darby bovine kidney cells: Optimization of culture conditions using palmitate. J. Dairy Sci. 92, 2027-2037 (2009).

    Aleshin, S., Grabeklis, S., Hanck, T., et al. Peroxisome proliferator-activated receptor (PPAR)-g positively controls and PPARa negatively controls cyclooxygenase-2 expression in rat brain astrocytes through a convergence on PPARb/d via mutual control of PPAR expression levels. Mol. Pharmacol. 76(2), 414-424 (2009).