An autophagy inhibitor
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Spautin-1

Item No. 17769

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
6-fluoro-N-[(4-fluorophenyl)methyl]-4-quinazolinamine
CAS Number
1262888-28-7
Molecular Formula
C15H11F2N3
Formula Weight
271.3
Purity
≥95%
Formulation
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMF:PBS(pH 7.2)(1:1): 0.5 mg/mlDMSO: 15 mg/mlEthanol: 10 mg/ml
λmax
225, 283, 323, 336 nm
SMILES
FC1=CC(C(NCC2=CC=C(F)C=C2)=NC=N3)=C3C=C1
InChi Code
InChI=1S/C15H11F2N3/c16-11-3-1-10(2-4-11)8-18-15-13-7-12(17)5-6-14(13)19-9-20-15/h1-7,9H,8H2,(H,18,19,20)
InChi Key
AWIVHRPYFSSVOG-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $36

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    5 mg

    $86

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    10 mg

    $153

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    50 mg

    $667

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    Product Description

    Spautin-1 is an autophagy inhibitor, inducing cell death in human breast cancer Bcap-37 cells grown in glucose-free media (EC50 = ~1.25 µM).1 It does not significantly affect the viability of Bcap-37 cells grown in complete media. Spautin-1 promotes the degradation of Vps34 PI3K complex by inhibiting the deubiquitinating enzymes USP10 and USP13 (IC50s = 0.58 and 0.69 µM, respectively).1 It is used to elucidate the role of autophagy in cellular processes.2,3 Spautin-1 has also been used to demonstrate roles for autophagy in viral infection.4,5

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Liu, J., Xia, H., Kim, M., et al. Beclin1 controls the levels of p53 by regulating the deubiquitination activity of USP10 and USP13. Cell 147, 223-234 (2011).

    2. Salabei, J.K., Cummins, T.D., Singh, M., et al. PDGF-mediated autophagy regulates vascular smooth muscle cell phenotype and resistance to oxidative stress. Biochem. J. 451(3), 375-388 (2013).

    3. Vakifahmetoglu-Norberg, H., Kim, M., Xia, H.-G., et al. Chaperone-mediated autophagy degrades mutant p53. Genes Dev. 27(15), 1718-1730 (2013).

    4. Mateo, R., Nagamine, C.M., Spagnolo, J., et al. Inhibition of cellular autophagy deranges dengue virion maturation. J. Virol. 87(3), 1312-1321 (2013).

    5. Bouley, S.J., Maginnis, M.S., Derdowski, A., et al. Host cell autophagy promotes BK virus infection. Virology 456-457, 87-95 (2014).