For immunochemical detection of citrullinated histone H3
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Histone H3 (Citrullinated R2 + R8 + R17) Polyclonal Antibody

Item No. 17855

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Immunogen
Histone H3 peptide with citrullinations at R2, R8, and R17
Formulation (Request formulation change)
500 µl of peptide affinity-purified polyclonal antibody
Storage Buffer
TBS, pH 7.4, with 50% glycerol, 0.1% BSA, and 0.02% sodium azide
Host
Rabbit
Applications
ELISA, WB
Cross Reactivity
(-) Non-citrullinated H3
Species Reactivity
(+) Human; other species not tested
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea (500 µl)

    $534

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    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications, including citrullination. Histone H3 is subject to citrullination of arginine residues by peptidyl arginine deiminase 4 (PAD4) at positions 2, 8, 17 and 26, and this citrullination blocks methylation of these residues by protein-arginine methyltransferase 4 (PRMT4) and induces transcriptional repression of target genes. Citrullinated histone H3 is a component of neutrophil extracellular traps (NETs) that acts as an autoantigen to induce production of anti-citrullinated protein antibodies associated with various diseases such as sepsis, multiple sclerosis, rheumatoid arthritis, and multiple myeloma.1,2,3,4 Cayman’s Histone H3 (Citrullinated R2 + R8 + R17) Polyclonal Antibody can be used for ELISA and Western blot applications. The antibody recognizes citrullinated histone H3 at 16 kDa from human samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Xu, Y.-M., Du, J.-Y., and Lau, A.T.Y. Posttranslational modifications of human histone H3: An update. Proteomics 14(17-18), 2047-2060 (2014).

    2. Li, Y., Liu, Z., Liu, B., et al. Citrullinated histone H3: A novel target for the treatment of sepsis. Surgery 156(2), 229-234 (2014).

    3. Moscarello, M.A., Mastronardi, F.G., and Wood, D.D. The role of citrullinated proteins suggests a novel mechanism in the pathogenesis of multiple sclerosis. Neurochem. Res. 32(2), 251-256 (2007).

    4. McNee, G., Eales, K.L., Wei, W., et al. Citrullination of histone H3 drives IL-6 production by bone marrow mesenchymal stem cells in MGUS and multiple myeloma. Leukemia 31(2), 373-381 (2017).