Host: E. coli • AA: 1-136 (full length) • MW: 15.5 kDa
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Histone H3 (human, recombinant; PAD4 citrullinated)

Item No. 17926

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • H3
  • H3C1
  • Histone H3.1
Purity
≥90% estimated by SDS-PAGE
Source
Recombinant human histone H3 expressed in E. coli and citrullinated by PAD4
Amino Acids
1-136 (full length)
MW
15.5 kDa
Formulation (Request formulation change)
A solution in PBS, pH 7.4
Host
E. coli
Applications
Immunoreactive with anti-citrulline antibodies by Western blot and ELISA
UniProt Accession №
P68431
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $600

    Add
    Cayman Chemical
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    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications, including citrullination. Histone H3 is subject to citrullination of arginine residues by peptidyl arginine deiminase 4 (PAD4) at positions 2, 8, 17, and 26, and this citrullination blocks methylation of these residues by protein-arginine methyltransferase 4 (PRMT4) and induces transcriptional repression of target genes. Citrullinated histone H3 is a component of neutrophil extracellular traps (NETs) that acts as an autoantigen to induce the production of anti-citrullinated protein antibodies associated with various diseases such as sepsis, multiple sclerosis, rheumatoid arthritis, and multiple myeloma.1,2,3,4 Cayman's Citrullinated Histone H3 (human, recombinant) protein is derived from purified Histone H3 (human, recombinant) (Item No. 10263) that has been modified by PAD4, which is removed by affinity chromatography after the reaction.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Xu, Y.-M., Du, J.-Y., and Lau, A.T.Y. Posttranslational modifications of human histone H3: An update. Proteomics 14(17-18), 2047-2060 (2014).

    2. Li, Y., Liu, Z., Liu, B., et al. Citrullinated histone H3: A novel target for the treatment of sepsis. Surgery 156(2), 229-234 (2014).

    3. Moscarello, M.A., Mastronardi, F.G., and Wood, D.D. The role of citrullinated proteins suggests a novel mechanism in the pathogenesis of multiple sclerosis. Neurochem. Res. 32(2), 251-256 (2007).

    4. McNee, G., Eales, K.L., Wei, W., et al. Citrullination of histone H3 drives IL-6 production by bone marrow mesenchymal stem cells in MGUS and multiple myeloma. Leukemia 31(2), 373-381 (2017).

    Product Citations

    Moon, J.-S., Younis, S., Ramadoss, N.S., et al. Cytotoxic CD8+ T cells target citrullinated antigens in rheumatoid arthritis. Nat. Commun. 14(1), 319 (2023).

    Surolia, R., Li, F.J., Wang, Z., et al. NETosis in the pathogenesis of acute lung injury following cutaneous chemical burns. JCI Insight 6(10), e147564 (2021).