A fluorogenic dye
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Calcein Blue AM

Item No. 20637

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-N-[(7-hydroxy-4-methyl-2-oxo-2H-1-benzopyran-8-yl)methyl]-glycine, (acetyloxy)methyl ester
CAS Number
168482-84-6
Synonyms
  • Calcein Blue Acetoxymethyl ester
Molecular Formula
C21H23NO11
Formula Weight
465.4
Purity
≥90%
Emission
445 nm
Excitation
360 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: Soluble
SMILES
OC1=CC=C(C(C)=CC(O2)=O)C2=C1CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O
InChi Code
InChI=1S/C21H23NO11/c1-12-6-18(26)33-21-15(12)4-5-17(25)16(21)7-22(8-19(27)31-10-29-13(2)23)9-20(28)32-11-30-14(3)24/h4-6,25H,7-11H2,1-3H3
InChi Key
GSFJGCPBBHLSPH-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    1 mg

    $143

    Add
    Product Description

    Calcein Blue AM is a fluorogenic dye that is used to assess cell viability. As the acetoxymethyl ester, Calcein Blue AM is non-fluorescent but cell-permeant and is hydrolyzed to the fluorescent probe Calcein Blue (Item No. 20636) by intracellular esterases. Calcein Blue is retained by living cells and displays excitation/emission maxima of 360/445 nm, respectively. Calcein Blue AM is a blue-emitting variant of the green fluorophore Calcein AM (Item No. 14948), which has been used to evaluate cell viability and cytotoxicity.1,2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Bratosin, D., Mitrofan, L., Palii, C., et al. Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging. Cytometry 66(1), 78-84 (2005).

    2. Lévesque, A., Paquet, A., and Pagé, M. Measurement of tumor necrosis factor activity by flow cytometry. Cytometry 20(2), 181-184 (1995).