For immunochemical detection of His tags on recombinant proteins
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Anti-His-tag Rabbit Monoclonal Antibody (Clone RM146)

Item No. 20725

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Immunogen
Mixture of a peptide with 6xHis-tag at the N-terminus and another peptide with 6xHis-tag at the C-terminus
Clone Designation
RM146
Formulation (Request formulation change)
100 µg of protein A affinity-purified antibody from an animal origin–free culture supernatant
Storage Buffer
50% glycerol/PBS with 1% BSA and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
WB, FC, ICC, IHC, and IP
Cross Reactivity
(+) Recombinant proteins containing the 6xHis-tag(+) 10xHis-tag fused to either the amino or carboxy terminus(-) Other endogenous protein in mammalian cells or bacteria
Species Reactivity
(+) All species
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µg

    $438

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    Product Description

    Anti-His-tag Rabbit Monoclonal Antibody (Clone RM146) is a probe for immunochemical detection of His tags on recombinant proteins by western blot, immunoprecipitation, immunohistochemistry, immunocytochemistry, or flow cytometry. Recombinant proteins are routinely labeled with affinity tags, such as poly-histidine (poly-His), to facilitate both their detection and purification. Poly-His tags are often the tag of choice due to their small size, less potential to interfere in protein folding, weak immunogenicity, and high affinity for Ni2+.

    WARNING This product is not for human or veterinary use.