For immunochemical detection of His-tagged proteins
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His-tag Monoclonal Antibody (Clone RMH01)

Item No. 32187

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Deca-His Tag
  • Decahistidine Tag
  • Hexa-His Tag
  • Hexahistidine Tag
  • Poly-His Tag
Immunogen
Mixture of a peptide with a 6x His tag at the N-terminus and a peptide with a 6x His tag at the C-terminus
Clone Designation
RMH01
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Chimeric Monoclonal Antibody
Isotype
IgG
Applications
ELISA, WB
Cross Reactivity
(+) His-tagged proteins(-) Endogenous mammalian or bacterial proteins
Species Reactivity
(Independent) Species
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µg

    $438

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    Product Description

    His-tag Monoclonal Antibody is a probe for the immunochemical detection of histidine tags on recombinant proteins. Recombinant proteins are commonly labeled with affinity tags, such as 6-10 histidine residues (6x-10x His), to facilitate both their detection and purification.1 Poly-His tags are commonly utilized because of their small size, low potential to interfere in protein folding or activity, weak immunogenicity, and high affinity for transition metal ion matrices, such as Ni2+, used in immobilized metal-affinity chromatography (IMAC) for protein purification.2,1 Cayman’s His-tag Monoclonal Antibody (Clone RMH01) is comprised of rabbit IgG variable domains and the human IgG1 constant domain. This antibody can be used for ELISA and Western blot (WB) applications. It recognizes proteins containing 6x or 10x His tags fused to either the N- or C-terminus and is recognized by anti-human IgG secondary antibodies.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Terpe, K. Overview of tag protein fusions: From molecular and biochemical fundamentals to commercial systems. Appl. Microbiol. Biotechnol. 60(5), 523-533 (2003).

    2. Spriestersbach, A., Kubicek, J., Schäfer, F., et al. Purification of His-tagged proteins. Methods in Enzymology 559, 1-15 (2015).