For immunochemical detection of His-tagged proteins
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His-tag Recombinant Monoclonal Antibody - Biotinylated

Item No. 35732

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Hexa-His Tag
  • Hexahistidine Tag
  • 6X His Tag
Immunogen
This antibody, which contains a mouse IgG2a isoform, was produced as a recombinant protein expressed in HEK293-derived cells using an engineered construct based on a monoclonal anti-His antibody
Formulation (Request formulation change)
100 µg of biotinylated protein G-purified monoclonal antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
HEK293 cells
Isotype
IgG2a
Applications
ELISA, WB
Cross Reactivity
(+) His-tagged proteins(-) GST-tagged proteins
Species Reactivity
(Independent) Species
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µg

    $440

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    Product Description

    His-tag Recombinant Monoclonal Antibody - Biotinylated is a probe for the immunochemical detection of histidine tags on recombinant proteins. Recombinant proteins are commonly labeled with affinity tags, such as six histidine residues (6x-His), to facilitate both their detection and purification.1 Poly-His tags are commonly utilized because of their small size, low potential to interfere in protein folding or activity, weak immunogenicity, and high affinity for transition metal ion matrices, such as Ni2+, used in immobilized metal-affinity chromatography (IMAC) for protein purification.1,2 Cayman's His-tag Recombinant Monoclonal Antibody - Biotinylated was produced as a recombinant protein using an engineered construct based on a monoclonal anti-His antibody. This antibody can be used for ELISA and Western blot (WB) applications. It recognizes proteins containing internal, N-, or C-terminal 6x-His tags and is recognized by anti-mouse IgG secondary antibodies.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Terpe, K. Overview of tag protein fusions: From molecular and biochemical fundamentals to commercial systems. Appl. Microbiol. Biotechnol. 60(5), 523-533 (2003).

    2. Spriestersbach, A., Kubicek, J., Schäfer, F., et al. Purification of His-tagged proteins. Methods in Enzymology 559, 1-15 (2015).