A fluorescent substrate of β-glucosidase and β-glucocerebrosidase
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4-Methylumbelliferyl-β-D-Glucopyranoside

Item No. 20948

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
7-(β-D-glucopyranosyloxy)-4-methyl-2H-1-benzopyran-2-one
CAS Number
18997-57-4
Synonyms
  • 4-MU-GLU
  • 4-MU-β-Gluc
  • 4-MUG
  • MU-GLU
Molecular Formula
C16H18O8
Formula Weight
338.3
Purity
≥98%
Emission
445-454 nm
Excitation
330 nm (pH 4.6), 370 nm (pH 7.4), 385 nm (pH 10.4)
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 5 mg/mlDMSO: 50 mg/ml
λmax
317 nm
SMILES
OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](OC2=CC=C(C(C)=CC(O3)=O)C3=C2)O1
InChi Code
InChI=1S/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13-,14+,15-,16-/m1/s1
InChi Key
YUDPTGPSBJVHCN-YMILTQATSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    250 mg

    $36

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    500 mg

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    1 g

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    5 g

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    Product Description

    4-Methylumbelliferyl-β-D-Glucopyranoside (4-MUG) is a fluorogenic substrate of β-glucosidase and β-glucocerebrosidase (also known as glucosylceramidase). In addition to its use in characterizing novel β-glucosidases, 4-MUG is used in assays to evaluate deficiency in β- glucocerebrosidase activity related to Gaucher disease.1,2,3 Hydrolysis of 4-MUG releases the fluorescent product 4-MU, which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively.4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hernández-Guzmán, A., Flores-Martínez, A., Ponce-Noyola, P., et al. Purification and characterization of an extracellular β-glucosidase from Sporothrix schenckii. FEBS Open Bio. 6(11), 1067-1077 (2016).

    2. Kwapiszewski, R., Skolimowski, M., Ziółkowska, K., et al. A microfluidic device with fluorimetric detection for intracellular components analysis. Biomed Microdevices 13(3), 431-440 (2011).

    3. Shanmuganathan, M.K., and Britz-McKibbin, P. Inhibitor screening of pharmacological chaperones for lysosomal β-glucocerebrosidase by capillary electrophoresis. Anal. Bioanal. Chem. 399(8), 2843-2853 (2011).

    4. Zhi, H., Wang, J., Wang, S., et al. Fluorescent properties of hymecromone and fluorimetric analysis of hymecromone in compound dantong capsule. J. Spectrosc. 147128 (2013).